Protein Info for PS417_03965 in Pseudomonas simiae WCS417

Updated annotation (from data): fructose-specific PTS system (fructose 1-phosphate forming), EI, Hpr, and EII-A components
Rationale: Specifically important for utilizing fructose and mannose. The phenotype on mannose is not explained.
Original annotation: PTS fructose transporter subunit IIA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 952 transmembrane" amino acids 587 to 610 (24 residues), see Phobius details PF00359: PTS_EIIA_2" amino acids 4 to 140 (137 residues), 94.5 bits, see alignment E=1.5e-30 PF00381: PTS-HPr" amino acids 286 to 366 (81 residues), 57.2 bits, see alignment E=3.5e-19 PF05524: PEP-utilisers_N" amino acids 403 to 518 (116 residues), 83.9 bits, see alignment E=2.6e-27 TIGR01417: phosphoenolpyruvate-protein phosphotransferase" amino acids 404 to 950 (547 residues), 485.8 bits, see alignment E=7.9e-150 PF00391: PEP-utilizers" amino acids 544 to 615 (72 residues), 76 bits, see alignment 3.5e-25 PF02896: PEP-utilizers_C" amino acids 644 to 924 (281 residues), 358.1 bits, see alignment E=8.6e-111

Best Hits

KEGG orthology group: K02768, PTS system, fructose-specific IIA component [EC: 2.7.1.69] K08483, phosphotransferase system, enzyme I, PtsI [EC: 2.7.3.9] K11183, phosphocarrier protein FPr (inferred from 97% identity to pfs:PFLU0804)

Predicted SEED Role

"Phosphoenolpyruvate-protein phosphotransferase of PTS system (EC 2.7.3.9)" in subsystem Fructose and Mannose Inducible PTS or Fructose utilization or Mannitol Utilization (EC 2.7.3.9)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.1.69, 2.7.3.9

Use Curated BLAST to search for 2.7.1.69 or 2.7.3.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7TUS7 at UniProt or InterPro

Protein Sequence (952 amino acids)

>PS417_03965 fructose-specific PTS system (fructose 1-phosphate forming), EI, Hpr, and EII-A components (Pseudomonas simiae WCS417)
MLELTVEQISMGQVAVDKSAALHLLAEKLVADGLVAEGYLSGLQAREAQGSTFLGQGIAI
PHGTPETRDQVFSTGVRLLQFPEGVDWGDGQIVYLAIGIAAKSDEHLRLLQLLTRALGET
DLGQALRRAGSAEALLKLLQGAPQELALDAQMIGLGVSADDFEELVWRGARLLRQADCVS
NGFAAVLQQVDALPLGDGLWWLHSEQTVKRPGLAFVTPDKPMRYLGQPLNGLFCLASLGE
AHQTLLERLCALLIEGRGQELGRATSSRAVLEVLGGELPPDWPAARITLANAHGLHARPA
KILAQLAKSFDGDLRVRIVDGPVGAVSVKSLSKLLSLGARRGQVLEFIAEPSIAGDALPA
LLAAVEEGLGEDVEPLPTLSVQPEVLDIEPELSAPLAGSQVQAIAAAPGIAIGPAHIQVL
QVFDYPLRGESCAIERERLHSALADVRRDIQGLIERSQSKAIREIFVTHQEMLDDPELTD
EVDTRLKQGESAEAAWMSVIEAAAKQQESLQDALLAERAADLRDIGRRVLAQLCGVETSQ
EPSEPYILVMDEVGPSDVARLDPARVAGILTARGGATAHSAIVARALGIPALVGAGPAVL
LLAAGTPLLLDGQRGRLHVDADAATLQRATVERDTREQRLQAASAQRHEPALTRDGHAVE
VFANIGESAGVASAVEQGAEGIGLLRTELIFMAHPQAPDEATQEAEYRRVLDGLAGRPLV
VRTLDVGGDKPLPYWPIAEEENPFLGVRGIRLTLQRPQIMEAQLRALLRSADNRPLRIMF
PMVGSVDEWRAARDMTERLRLEIPVADLQLGIMIEVPSAALLAPVLAKEVDFFSVGTNDL
TQYTLAIDRGHPTLSAQADGLHPAVLQLIDITVRAAHAHGKWVGVCGELAADPLAVPVLV
GLGVDELSVSARSIPEVKARVREFSLSEAQGLAQKALAVGSPAEVRALVEAV