Protein Info for GFF771 in Variovorax sp. SCN45

Annotation: Malto-oligosyltrehalose trehalohydrolase (EC 3.2.1.141)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 622 PF02922: CBM_48" amino acids 8 to 72 (65 residues), 33.9 bits, see alignment E=5.1e-12 TIGR02402: malto-oligosyltrehalose trehalohydrolase" amino acids 20 to 554 (535 residues), 696.8 bits, see alignment E=8.1e-214 PF00128: Alpha-amylase" amino acids 131 to 214 (84 residues), 41.8 bits, see alignment E=1.4e-14 PF11941: DUF3459" amino acids 519 to 613 (95 residues), 31.5 bits, see alignment E=2.8e-11

Best Hits

KEGG orthology group: K01236, maltooligosyltrehalose trehalohydrolase [EC: 3.2.1.141] (inferred from 76% identity to vpe:Varpa_3008)

Predicted SEED Role

"Malto-oligosyltrehalose trehalohydrolase (EC 3.2.1.141)" in subsystem Trehalose Biosynthesis (EC 3.2.1.141)

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.2.1.141

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (622 amino acids)

>GFF771 Malto-oligosyltrehalose trehalohydrolase (EC 3.2.1.141) (Variovorax sp. SCN45)
MIRAHRMPFGATVNDGGGVAFSLWAPSAGDVRLEHRPVGGENSTHPMARDAQGWHTLTLP
EAAHGDSYRFHLPDGTAVPDPASRFNPEDVHGPSVVIDPRHHHWRDDAWRGRPWEEAVVY
ELHIGAFTAEGTFNAARERLGDLAELGVTAIELMPLADFPGRRNWGYDGVLQFAPDASYG
TPDELKALVDAAHGLGLMVLLDVVYNHFGPEGNYLHAYCPEFFNPAHRTPWGSAINFDGE
GARTVRDFFIHNALYWVEEYRFDGLRMDAIHAISDSSHPHIVQEIREALQAGPGRERHIH
LVLENDANQASMLARDGHGMPVAGTAQWNDDLHHAVHVLATGERDGYYADYADDPVCRFA
CALAEGFVYQGQPSAFRNGERRGEPSTRLPSQAFVSFLQTHDQVGNRAFGERLHALGDPV
LTRAAMACLLLSPHVPMLFMGDEFAASTPFLYFCDFGPELAQAVSDGRRSEFGGFAAFKD
EAARARIPDPNAESTFLASKLNWRERGSRTHFARFSEVQQLLELRRRLIVPHLAGSTGAG
VYLCENGTLRVHWDLGPPQEGREGRTAVRLHLLAHFGDGPAAAALAPPGALVYSSGVQHA
EPGALLLERGAVLATLEGMHGG