Protein Info for GFF768 in Sphingobium sp. HT1-2

Annotation: Putative formate dehydrogenase oxidoreductase protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01701: oxidoreductase alpha (molybdopterin) subunit
  • amino acids 23 to 753 (731 residues), 960.6 bits, E=2.8e-293
• PF00384: Molybdopterin
  • amino acids 119 to 495 (377 residues), 85.6 bits, E=3.6e-28
• PF01568: Molydop_binding
  • amino acids 644 to 747 (104 residues), 59.2 bits, E=3.8e-20

Best Hits

Swiss-Prot: 59% identical to YDEP_ECOLI: Protein YdeP (ydeP) from Escherichia coli (strain K12)

KEGG orthology group: K00123, formate dehydrogenase, alpha subunit [EC: 1.2.1.2] (inferred from 67% identity to msl:Msil_2417)

Predicted SEED Role

"Putative formate dehydrogenase oxidoreductase protein" in subsystem Formate hydrogenase

MetaCyc Pathways

• formate oxidation to CO₂ (1/1 steps found)
• oxalate degradation VI (1/4 steps found)
• purine nucleobases degradation II (anaerobic) (15/24 steps found)
• oxalate degradation III (1/5 steps found)
• superpathway of C1 compounds oxidation to CO₂ (5/12 steps found)
• purine nucleobases degradation I (anaerobic) (5/15 steps found)

KEGG Metabolic Maps

• Glyoxylate and dicarboxylate metabolism
• Methane metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.2.1.2

Use Curated BLAST to search for 1.2.1.2

Protein Sequence (755 amino acids)

>GFF768 Putative formate dehydrogenase oxidoreductase protein (Sphingobium sp. HT1-2)
MDKAAEDLIPDTVEGVSSYDGPAGGWGALRAVAKTVAEQMRASPDTRALLQMNQPDGFDC
PGCAWPDPKHTSSFEFCENGAKAVTWEATVKRVDPDFFARHSVSELWDWTDHKLEDAGRL
TEPLRYDAATDHFAPIGWDEAFARAGAALQALDHADQAEFYCSGRASNESAFLYQLFARE
FGTNNFPDCSNMCHEATSTGLPKSIGIGKGTVTLEDFDHADAIFCIGHNPGTNHPRMLST
LAAASKRGVPIIVANPMRERGLERFKSPQHPTEMLSPGATPLASAYHQVRIGGDMAMLKG
MMKALLAIEAQQPGTLDHEFIAQHTEGFDALRADIEATQWPWIERLSGLTREAIESMTHV
YARADRVIICYGMGITQHRHGTQNVQALANLLLLRGNFGKPGAGICPLRGHSNVQGDRTV
GITELPTEAMLQRLDTAFSIQSPRKHGHNAVEALAAMHAGESKALISLGGNLAVAMPDPD
ACFAAVRRLDLSVNILTKFNRTCLLTARETLVLPCLGRTELDLQAAGPQWVTVEDSMSMV
HASRGKLKPPSPHVRSEPAIVAGLAKAAMPASRTDWDGMVADYDRIRDGIEAVYPDFRDF
NIRVREPGGFRLTIGPSDRIWNTPSGKAQFITHPAAPDEGALPLLLTTIRSHDQYNTTIY
SYDDRYRGIHGRRDIIFANEIDLAEMGLAHGDKVDVTTPAGRELRGFTIVMHAIARGSLA
AYYPEANNLVPLDDYDPASGTPAYKSIPVKVRAAR