Protein Info for GFF763 in Sphingobium sp. HT1-2

Annotation: Glutamate synthase [NADPH] small chain (EC 1.4.1.13)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01318: glutamate synthase, small subunit
  • amino acids 9 to 470 (462 residues), 515.7 bits, E=5e-159
• PF14691: Fer4_20
  • amino acids 26 to 136 (111 residues), 124.2 bits, E=7.6e-40
• PF01494: FAD_binding_3
  • amino acids 150 to 182 (33 residues), 22.3 bits, see alignment (E = 2.6e-08)
• PF07992: Pyr_redox_2
  • amino acids 151 to 458 (308 residues), 108.1 bits, E=2e-34
• PF13450: NAD_binding_8
  • amino acids 153 to 188 (36 residues), 37.4 bits, 9e-13
• PF00070: Pyr_redox
  • amino acids 292 to 366 (75 residues), 23.7 bits, E=2e-08

Best Hits

Swiss-Prot: 68% identical to GLTD_AZOBR: Glutamate synthase [NADPH] small chain (gltD) from Azospirillum brasilense

KEGG orthology group: K00266, glutamate synthase (NADPH/NADH) small chain [EC: 1.4.1.13 1.4.1.14] (inferred from 92% identity to sjp:SJA_C1-02420)

MetaCyc: 68% identical to glutamate synthase beta subunit (Azospirillum brasilense)
Glutamate synthase (NADPH). [EC: 1.4.1.13]

Predicted SEED Role

"Glutamate synthase [NADPH] small chain (EC 1.4.1.13)" in subsystem Ammonia assimilation or Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis (EC 1.4.1.13)

MetaCyc Pathways

• L-asparagine biosynthesis III (tRNA-dependent) (4/4 steps found)
• glutaminyl-tRNA^gln biosynthesis via transamidation (4/4 steps found)
• ammonia assimilation cycle III (3/3 steps found)
• L-glutamate biosynthesis I (2/2 steps found)
• ammonia assimilation cycle I (2/2 steps found)
• L-glutamate biosynthesis IV (1/1 steps found)
• L-glutamine degradation I (1/1 steps found)
• L-glutamine degradation II (1/1 steps found)
• L-citrulline biosynthesis (6/8 steps found)
• superpathway of ammonia assimilation (plants) (2/3 steps found)
• superpathway of L-citrulline metabolism (8/12 steps found)
• L-glutamate and L-glutamine biosynthesis (4/7 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Glutamate metabolism
• Nitrogen metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.4.1.13

Use Curated BLAST to search for 1.4.1.13 or 1.4.1.14

Protein Sequence (483 amino acids)

>GFF763 Glutamate synthase [NADPH] small chain (EC 1.4.1.13) (Sphingobium sp. HT1-2)
MAENPMLKFVGKEQAYPEKRGADARAEDFLEISKSFILDKAEEQASRCSQCGVPYCSTHC
PLHNHIPDWLRLTAEGRLREAYELSNLTSTMPEICGRICPQDRLCEGNCVIEFSGHGSVT
IGSVEKFITDTAWKEGWVEPLVPGAARGQSVGVIGAGPAGLTTAEYLRVAGYEVHVYDRH
DRAGGLLTYGIPGFKLEKDVVMRRVQRLKDGGIIFHEGFEVGRDASMEQLRAKHDAILIA
TGVYKARDIKAPGVGAPGVVKALDYLTASNKAGFGDAVPGHEDGTLLATGKNVVVIGGGD
TAMDCVRTAIRQGAKSVKCLYRRDRDNMPGSQREVANAEEEGVEFVWLTAPIAFEGTEHV
TGVKVTKMRLGSPDASGRRAPEPDPGSEHTLEADLVIKALGYDPEDLPRLFGADDLSVTR
WGTLRVDHKTMMTSMDGVFAAGDIVRGASLVVWAIRDGRDVTDHMHKYLKAKAVAAASEK
VAA