Protein Info for GFF758 in Variovorax sp. SCN45

Annotation: 2-Keto-3-deoxy-D-manno-octulosonate-8-phosphate synthase (EC 2.5.1.55)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 281 PF00793: DAHP_synth_1" amino acids 9 to 274 (266 residues), 229.4 bits, see alignment E=2e-72 TIGR01362: 3-deoxy-8-phosphooctulonate synthase" amino acids 17 to 274 (258 residues), 380.3 bits, see alignment E=1.8e-118

Best Hits

Swiss-Prot: 83% identical to KDSA_CHRVO: 2-dehydro-3-deoxyphosphooctonate aldolase (kdsA) from Chromobacterium violaceum (strain ATCC 12472 / DSM 30191 / JCM 1249 / NBRC 12614 / NCIMB 9131 / NCTC 9757)

KEGG orthology group: K01627, 2-dehydro-3-deoxyphosphooctonate aldolase (KDO 8-P synthase) [EC: 2.5.1.55] (inferred from 87% identity to vpe:Varpa_4619)

MetaCyc: 75% identical to 3-deoxy-D-manno-octulosonate 8-phosphate synthase (Escherichia coli K-12 substr. MG1655)
3-deoxy-8-phosphooctulonate synthase. [EC: 2.5.1.55]

Predicted SEED Role

"2-Keto-3-deoxy-D-manno-octulosonate-8-phosphate synthase (EC 2.5.1.55)" in subsystem KDO2-Lipid A biosynthesis (EC 2.5.1.55)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.5.1.55

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (281 amino acids)

>GFF758 2-Keto-3-deoxy-D-manno-octulosonate-8-phosphate synthase (EC 2.5.1.55) (Variovorax sp. SCN45)
LTTVTITPNVHVRNDGPFVLFGGVNVLESKDLALRSAEEYVRVTGKLGIPYVFKGSFDKA
NRSSIHSYRGPGLDEGLKILQAVKDAFGVPVITDVHEIAQAAPVAEVVDVLQLPAFLARQ
TDLVVALAKTGRAINIKKPQFLSPPQMLNIVEKFKEAGNDRLILCDRGTCFGYDNLVVDM
LGFGVMKKVTQNLPIIFDVTHALQQREAGAAASGGRREQVADLARAGMAVGLAGLFLEAH
PDPAQAKCDGPSALPLDKLEPFLAQIKALDDLVKSFAPLNI