Protein Info for GFF754 in Sphingobium sp. HT1-2

Annotation: Carboxyl-terminal protease (EC 3.4.21.102)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 451 signal peptide" amino acids 1 to 27 (27 residues), see Phobius details PF22694: CtpB_N-like" amino acids 30 to 80 (51 residues), 42.3 bits, see alignment 2.2e-14 TIGR00225: C-terminal processing peptidase" amino acids 47 to 371 (325 residues), 349.5 bits, see alignment E=8.8e-109 PF00595: PDZ" amino acids 93 to 162 (70 residues), 32.8 bits, see alignment E=1.8e-11 PF13180: PDZ_2" amino acids 95 to 175 (81 residues), 46.3 bits, see alignment E=1.1e-15 PF17820: PDZ_6" amino acids 114 to 164 (51 residues), 48.8 bits, see alignment 1.2e-16 PF03572: Peptidase_S41" amino acids 192 to 358 (167 residues), 178.4 bits, see alignment E=2.2e-56

Best Hits

Swiss-Prot: 49% identical to CTPA_BARBK: Carboxy-terminal-processing protease (ctpA) from Bartonella bacilliformis (strain ATCC 35685 / NCTC 12138 / KC583)

KEGG orthology group: K03797, carboxyl-terminal processing protease [EC: 3.4.21.102] (inferred from 93% identity to sjp:SJA_C1-28780)

Predicted SEED Role

"Carboxyl-terminal protease (EC 3.4.21.102)" (EC 3.4.21.102)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.4.21.102

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (451 amino acids)

>GFF754 Carboxyl-terminal protease (EC 3.4.21.102) (Sphingobium sp. HT1-2)
MKSTFLQGAVALGALALIPATTAALADGEASSYKALDEFMDVFQKVRSDYVEKVDDEKLI
KGAIDGMLASLDPHSSFLDARDFQNLRTQTEGSYGGLGLSVTQEDGAVKVIAPTQDTPAW
RAGIKAGDYITHIDGQLIYGGTLDEAVDKMRGAPGTSIKLTIVRTGRDKPIDLTLTREII
EIKPVKWEVKNNIGVINIVSFSANTGADVRSAIRSIDKSLGHKPTGYILDLRSNPGGLLD
EAVSTSDVFLERGEIVSQRGRNKGDVERYYAKPGDDAKGLPVIVLVDAGSASASEIVAGA
LQDQHRALIMGERSFGKGSVQTMLPLSNTTALKLTTARYFTPSGRSVQEGGIQPDIKVPQ
LSDPDYKDRPKFRESDLRRHLINEIKTDDAALEEDSKEDPRFAQTAEELKKKGIEDFQLD
YALKTISRLAGTPGATIAQAQQAAARKPGAK