Protein Info for GFF751 in Xanthobacter sp. DMC5

Annotation: DNA gyrase subunit A

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 919 TIGR01063: DNA gyrase, A subunit" amino acids 17 to 895 (879 residues), 1163.1 bits, see alignment E=0 PF00521: DNA_topoisoIV" amino acids 41 to 506 (466 residues), 531.1 bits, see alignment E=2.2e-163 PF03989: DNA_gyraseA_C" amino acids 539 to 584 (46 residues), 51 bits, see alignment 7.6e-18 amino acids 590 to 636 (47 residues), 23.9 bits, see alignment 2.3e-09 amino acids 649 to 690 (42 residues), 25.6 bits, see alignment 6.7e-10 amino acids 695 to 742 (48 residues), 37.1 bits, see alignment 1.7e-13 amino acids 798 to 844 (47 residues), 47.6 bits, see alignment 8.9e-17 amino acids 848 to 892 (45 residues), 42.2 bits, see alignment 4.3e-15

Best Hits

Swiss-Prot: 55% identical to GYRA_GRABC: DNA gyrase subunit A (gyrA) from Granulibacter bethesdensis (strain ATCC BAA-1260 / CGDNIH1)

KEGG orthology group: K02469, DNA gyrase subunit A [EC: 5.99.1.3] (inferred from 82% identity to azc:AZC_2328)

Predicted SEED Role

"DNA gyrase subunit A (EC 5.99.1.3)" in subsystem DNA topoisomerases, Type II, ATP-dependent or Resistance to fluoroquinolones (EC 5.99.1.3)

Isozymes

Compare fitness of predicted isozymes for: 5.99.1.3

Use Curated BLAST to search for 5.99.1.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (919 amino acids)

>GFF751 DNA gyrase subunit A (Xanthobacter sp. DMC5)
LADSETPKTGGEPPSDIRPVSITEELSRSYLDYAMSVIVARALPDVRDGLKPVHRRILFS
MHENGYEWNKQYRKSARVVGDVIGKYHPHGDQSVYDALVRMAQTFAMRVPLVDGQGNFGS
VDGDAPAAMRYTEVRLEKVTNTLLDDLDKETVDFQDNYDNSEHEPTVLPAKFPNLLVNGA
GGIAVGMATNIPPHNLGEIIDVTVALLDDPLLEPEALLDLLPGPDFPTGALILGRTGIRQ
AYLTGRGSILMRAKVAIEHIRKDREALIVTEIPYQVNKATMIEKIAELVRDKRVEGISEI
RDESDRDGMRVVIEIKRDAQADVVLNNLYRLTLLQTSFGVNMVALSGGKPGILTLHDVLT
AFIAFREEVISRRTKFLLRKARDRAHVLVGLAIAVANIDEVIRLIRTAPDPATARESLMG
RDWPAKDMAPLIALIADPRHKLKEDGTYRLSEVQARAILDLRLQRLTALGRDEIAEELDK
LAAEIADYLEILASRPRMRAIIRDELLAIKAEFGTPRRTEIVEGFGDLEDEDLIQREEMV
VTVSHAGYIKRVPLSTYRAQRRGGKGRSAMQTRDEDFVTRLFVASTHAPVLFFSSKGQVY
KMKVWRLPLAAPQARGKALINILPLEQDERITSVVLLPEDEGENQRLQIMFATTGGTVRR
NALTDFQQVNRNGKIAMKLAEGEAIADVGLCTEEDDVLLTTAAGACIRFPVTEVRLFKGR
DSVGVRGIRLEESDSVISMAIIRHVEADAEQRLAYIKMRRALRGEAGESAEVEQEEGASG
SGQLSQERYAEMGAKEQFILTVSENGYGKRTSSYEYRVTGRGGKGIVAMAVNERNGRLVA
SFPVEDADQIMLVTDGGQLIRCPVDGIRIVGRASQGVIVFDTAEGERVVSVEHISEDTGA
EGEAGESGVDAPGDDTPEA