Protein Info for GFF751 in Sphingobium sp. HT1-2

Annotation: 23S rRNA (pseudouridine(1915)-N(3))-methyltransferase (EC 2.1.1.177)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 140 PF02590: SPOUT_MTase" amino acids 1 to 137 (137 residues), 117.2 bits, see alignment E=3.4e-38

Best Hits

Swiss-Prot: 74% identical to RLMH_SPHWW: Ribosomal RNA large subunit methyltransferase H (rlmH) from Sphingomonas wittichii (strain RW1 / DSM 6014 / JCM 10273)

KEGG orthology group: K00783, hypothetical protein (inferred from 89% identity to sch:Sphch_1912)

Predicted SEED Role

"LSU m3Psi1915 methyltransferase RlmH" in subsystem Ribosome biogenesis bacterial

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (140 amino acids)

>GFF751 23S rRNA (pseudouridine(1915)-N(3))-methyltransferase (EC 2.1.1.177) (Sphingobium sp. HT1-2)
MLLHIIARGKIGRSPEADLVDRYVKRLTMPHKITEMPDRGGKLPPVGPGTVTVMLDEKGK
QLSSMDFARRIEGWRDTGTREIRFLIGAADGFDDAERTNADLLIAFGAMTWPHMIARAML
AEQLWRACSILANHPYHREG