Protein Info for GFF739 in Sphingobium sp. HT1-2
Annotation: GMP synthase [glutamine-hydrolyzing], amidotransferase subunit (EC 6.3.5.2) / GMP synthase [glutamine-hydrolyzing], ATP pyrophosphatase subunit (EC 6.3.5.2)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 81% identical to GUAA_ZYMMO: GMP synthase [glutamine-hydrolyzing] (guaA) from Zymomonas mobilis subsp. mobilis (strain ATCC 31821 / ZM4 / CP4)
KEGG orthology group: K01951, GMP synthase (glutamine-hydrolysing) [EC: 6.3.5.2] (inferred from 89% identity to eli:ELI_11710)MetaCyc: 54% identical to GMP synthase monomer (Saccharomyces cerevisiae)
GMP synthase (glutamine-hydrolyzing). [EC: 6.3.5.2]
Predicted SEED Role
"GMP synthase [glutamine-hydrolyzing] (EC 6.3.5.2)" in subsystem Purine conversions or Staphylococcal pathogenicity islands SaPI (EC 6.3.5.2)
MetaCyc Pathways
- superpathway of histidine, purine, and pyrimidine biosynthesis (42/46 steps found)
- superpathway of purine nucleotides de novo biosynthesis I (21/21 steps found)
- superpathway of purine nucleotides de novo biosynthesis II (23/26 steps found)
- superpathway of guanosine nucleotides de novo biosynthesis I (6/6 steps found)
- superpathway of guanosine nucleotides de novo biosynthesis II (7/8 steps found)
- L-asparagine biosynthesis III (tRNA-dependent) (4/4 steps found)
- glutaminyl-tRNAgln biosynthesis via transamidation (4/4 steps found)
- guanosine ribonucleotides de novo biosynthesis (4/4 steps found)
- superpathway of purine nucleotide salvage (11/14 steps found)
- ammonia assimilation cycle III (3/3 steps found)
- L-glutamate biosynthesis I (2/2 steps found)
- L-glutamine degradation I (1/1 steps found)
- L-citrulline biosynthesis (6/8 steps found)
- superpathway of L-citrulline metabolism (8/12 steps found)
- L-glutamate and L-glutamine biosynthesis (4/7 steps found)
KEGG Metabolic Maps
- Biosynthesis of alkaloids derived from histidine and purine
- Drug metabolism - other enzymes
- Glutamate metabolism
- Purine metabolism
Isozymes
Compare fitness of predicted isozymes for: 6.3.5.2
Use Curated BLAST to search for 6.3.5.2
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (523 amino acids)
>GFF739 GMP synthase [glutamine-hydrolyzing], amidotransferase subunit (EC 6.3.5.2) / GMP synthase [glutamine-hydrolyzing], ATP pyrophosphatase subunit (EC 6.3.5.2) (Sphingobium sp. HT1-2) MTLPLQDSILIVDFGSQVTQLIARRVREAGVYSEIAPFTQAQAAFDRMKPKGIILSGSPA GVPEEGSPRAPQALFDAGVPILGICYGQQVMSHQLGGEVRPGHETGEGGEFGRAFLTVTE PCALFDGLWEVGERHQVWMSHGDKVTKFAPGFKIVAVSDGAPFAVIADEERKFYGTQFHP EVVHTPDGGKLIANFVRHVCGLAGDWTMAEFRQVKIAEIREQVGTGRVICGLSGGVDSAV AAVLIHEAIGDQLTCVFVDHGLMRLGEAEQVVSLFREHYGIKLVHVNAEERFLGGLAGLT DPEKKRKFIGGEFIAVFEEEAAKIGGADFLAQGTLYPDVIESVSFTGGPSVTIKSHHNVG GLPERMNMKLVEPLRELFKDEVRVLGKELGLPDIFVGRHPFPGPGLAIRIPGEVTKERCD ILRKADFIYLEEIRNAGLYDAIWQAFAVLLPVRTVGVMGDYRTYDSVCAVRAVTSTDGMT ADIYPFDAAFLSRVATRIINEVKGINRVVYDYTSKPPGTIEWE