Protein Info for GFF7301 in Variovorax sp. SCN45
Annotation: Glycogen phosphorylase (EC 2.4.1.1)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: K00688, starch phosphorylase [EC: 2.4.1.1] (inferred from 96% identity to vpe:Varpa_0438)Predicted SEED Role
"Glycogen phosphorylase (EC 2.4.1.1)" in subsystem Glycogen metabolism or Maltose and Maltodextrin Utilization (EC 2.4.1.1)
MetaCyc Pathways
- glycogen degradation I (7/8 steps found)
- glycogen degradation II (5/6 steps found)
- sucrose biosynthesis II (6/8 steps found)
- starch degradation V (3/4 steps found)
- starch degradation III (2/4 steps found)
KEGG Metabolic Maps
Isozymes
No predicted isozymesUse Curated BLAST to search for 2.4.1.1
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (827 amino acids)
>GFF7301 Glycogen phosphorylase (EC 2.4.1.1) (Variovorax sp. SCN45) MTIKDFAYDHPDRDVAAFKRAVANKLIYAVGKDPVAASQDDWLNATSQAVRDQLVERWMM TTRANYAQDLKRVYYLSMEFLIGRTFTNALLAVDLYDTVREALADFGVDMDALAEREPDA ALGNGGLGRLAACFLDSMATLGVPGMGYGIRYEYGMFRQRIVDGQQVETPDYWLTRGNPW EFQRPEVNYRVRFGGHVQKREGANVPPGAVDWVDTHDVLAVAYDTIIPGYGTQATNTLRL WSARATEEIDLSAFNRGNYMGAVESKNQSENVSRVLYPDDSTPSGRELRLHQEYFFCSAS VQDLLRRYLRNHKTFDQLSEKVSIHLNDTHPVLAVPELMRLLLDQHGMPWDEAWAHTQKV FSYTNHTLMHEALETWPVEMLGRILPRHLQIIYDINARFLATVTQKLGNDVELMRRLSLV DEAGERRVRMAYVAVLASHSVNGVSGLHSELMKQSIFSDFAKLFPERFNNKTNGVTPRRW LAQANPPLAALLDQRIGKGWRRDLSQLEALKPMAVQPAFVRAFRHAKRENKLRLANWVEQ HLKIDLDTDAMFDVQVKRIHEYKRQLLNVLHVVARYHRILDAQAAGGAVDIVPRVVVFAG KAASAYVMAKLVIRLINDVASIVNADARVGKLLKVVFLPNYSVSLAEIIMPAADLSEQIS TAGTEASGTGNMKFALNGALTIGTLDGANVEMRENVGPENIFIFGNTAPEVADIRARGYQ PRDIYEGDAELKRVLDAIRDGAFSPGEPARYQGIYDALVNWGDHYLLLADYASYVAKQAE VDALYRDSDAWTRMAVLNVAGMGAFSSDRTIAQYAHEIWHTKPVVLG