Protein Info for GFF730 in Variovorax sp. SCN45

Annotation: Class A beta-lactamase (EC 3.5.2.6)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 296 signal peptide" amino acids 1 to 29 (29 residues), see Phobius details PF00144: Beta-lactamase" amino acids 42 to 287 (246 residues), 107.8 bits, see alignment E=6.7e-35 PF13354: Beta-lactamase2" amino acids 53 to 268 (216 residues), 142.7 bits, see alignment E=1.1e-45

Best Hits

Swiss-Prot: 57% identical to BLAC_YEREN: Beta-lactamase (blaA) from Yersinia enterocolitica

KEGG orthology group: K01467, beta-lactamase [EC: 3.5.2.6] (inferred from 78% identity to vpe:Varpa_2415)

Predicted SEED Role

"Beta-lactamase (EC 3.5.2.6)" in subsystem Beta-lactamase or Tn552 (EC 3.5.2.6)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.5.2.6

Use Curated BLAST to search for 3.5.2.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (296 amino acids)

>GFF730 Class A beta-lactamase (EC 3.5.2.6) (Variovorax sp. SCN45)
MNSPVVQRRTLLLAASVLPLAGACTAWSQKGPQASAEAQLAELERACGGRLGVVAFNTAS
GARLQHRPHERFPFCSTFKLMLSAAVLERSAKDASLLSRRVVYSRADIIPNSPVTEKHVA
DGMTVAALCAAAIQYSDNAAANLLMKVLGGPADVTAFARTIGDQAFRLDRWETELNTAIP
GDLRDTTTPAAMADSVQRLVLGDALGAAQRSQLQTWLLGNTTSAERFMAAVPPGWKVGDK
TGGGSYGTNNDVGVMWPPAGAPLVVSVYLTFPDKDAKTRNDVVASAARIAVSALAG