Protein Info for GFF7292 in Variovorax sp. SCN45

Annotation: ATP-dependent DNA helicase UvrD/PcrA, proteobacterial paralog

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 709 signal peptide" amino acids 1 to 18 (18 residues), see Phobius details PF13245: AAA_19" amino acids 48 to 299 (252 residues), 75.7 bits, see alignment E=1.2e-24 PF00580: UvrD-helicase" amino acids 48 to 313 (266 residues), 199.3 bits, see alignment E=3.1e-62 PF13361: UvrD_C" amino acids 320 to 474 (155 residues), 81.8 bits, see alignment E=2.1e-26 amino acids 584 to 641 (58 residues), 66.1 bits, see alignment 1.2e-21 PF13538: UvrD_C_2" amino acids 586 to 641 (56 residues), 41.2 bits, see alignment 3.7e-14

Best Hits

KEGG orthology group: K03657, DNA helicase II / ATP-dependent DNA helicase PcrA [EC: 3.6.4.12] (inferred from 92% identity to vpe:Varpa_0429)

Predicted SEED Role

"ATP-dependent DNA helicase UvrD/PcrA, proteobacterial paralog" in subsystem DNA repair, bacterial UvrD and related helicases

Isozymes

Compare fitness of predicted isozymes for: 3.6.4.12

Use Curated BLAST to search for 3.6.4.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (709 amino acids)

>GFF7292 ATP-dependent DNA helicase UvrD/PcrA, proteobacterial paralog (Variovorax sp. SCN45)
MQTAALPVFSTAPKTQAERLAAALASLNEAQRAAVEHGVGAAVGAGAQADQRPLLVIAGA
GSGKTSTLAHRVAHLIAGGVDPQRLLLLTFSRRAAQEMGRRAGQVLTKVLGLKSEAPPSL
PWAGTFHGIGARLLREYAAQIGLDENFTIHDRGDAEDLMGLARHELGLSSTVNRFPRKGT
CLSIYSRCVNTRAPLAEVLRESFPWCGEWEAELKTLFGAYVEAKHRQNVLDYDDLLLYWA
GMVAEPELAAHVGARFDHVLVDEYQDTNLLQASILLALKPDGRGVTVVGDDAQSIYSFRG
ATVRNILDFPSQFTQDARVVTLERNYRSTQPILDVSNRVIAQAAERHAKTLWTDKPSAGK
PLLVLVPDEAGQARWVADKVLAHREGGLALKSQAVLFRTSTHSAALELELARRNIPFVKF
GGLKFLEASHVKDLLAVLRFAQNPRGRMAGFRVTQLIPGIGPVTGTRLLDAMDQAADPVA
AVRDFVPPSAARAQWADFATAYEALRSPALEWPADVEVAMAWYQPHLERLYEDTSGVRRA
DVEQLARLAAGYGSRERFLTELTLDPPEATSDRPGPPLLDEDYLILSTIHSAKGQEWNSV
HVLNVVDGCLPADVAQSAHELEEERRLLYVAMTRARDHLHLLVPQRFYVTQQAVRGDRHL
YANRTRFITDADLAGFEQQTWPPPPERPPAVPPPTAVIDLRNRMRAAWR