Protein Info for PS417_03690 in Pseudomonas simiae WCS417

Annotation: NAD-dependent dehydratase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 299 TIGR01777: TIGR01777 family protein" amino acids 3 to 292 (290 residues), 357 bits, see alignment E=4.7e-111 PF01370: Epimerase" amino acids 3 to 221 (219 residues), 101.4 bits, see alignment E=1.1e-32 PF03446: NAD_binding_2" amino acids 8 to 64 (57 residues), 22.7 bits, see alignment E=1.7e-08 PF08338: DUF1731" amino acids 249 to 295 (47 residues), 65.7 bits, see alignment 5e-22

Best Hits

Swiss-Prot: 50% identical to YFCH_ECOLI: Epimerase family protein YfcH (yfcH) from Escherichia coli (strain K12)

KEGG orthology group: K07071, (no description) (inferred from 94% identity to pfs:PFLU0755)

Predicted SEED Role

"Cell division inhibitor"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7U6R2 at UniProt or InterPro

Protein Sequence (299 amino acids)

>PS417_03690 NAD-dependent dehydratase (Pseudomonas simiae WCS417)
MHILLTGGTGLIGRQLCQHWLAQGHRLTVWSRHPEQVAKLCGAQVAGVEHLQQVIGPVDA
VINLAGAPIADRPWTHKRKALLWSSRISLTETLLAWLEGLEQKPAVLISGSAVGWYGDGG
ERELTEASGPVQDDFPSQLCIAWEETAQRAEALGIRVVLVRTGLVLSAEGGFLSRLLLPF
KLALGGPIGTGRQWMPWVHIKDQIALIDFLLHKADASGPYNACAPHPVRNREFAKTLGRV
LHRPAFMPMPAFALKVGLGELSGLLLGGQKAVPERLLAAGFTFQFTELHAALDDVSSRL