Protein Info for GFF723 in Sphingobium sp. HT1-2

Annotation: Cyclopropane-fatty-acyl-phospholipid synthase (EC 2.1.1.79)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 413 PF02353: CMAS" amino acids 119 to 385 (267 residues), 291 bits, see alignment E=3.6e-90 PF01209: Ubie_methyltran" amino acids 160 to 319 (160 residues), 26.5 bits, see alignment E=1.6e-09 PF13489: Methyltransf_23" amino acids 175 to 288 (114 residues), 44.7 bits, see alignment E=5.1e-15 PF07021: MetW" amino acids 178 to 242 (65 residues), 24.9 bits, see alignment E=5.9e-09 PF13847: Methyltransf_31" amino acids 180 to 287 (108 residues), 47.2 bits, see alignment E=7.8e-16 PF13649: Methyltransf_25" amino acids 185 to 279 (95 residues), 66.2 bits, see alignment E=1.5e-21 PF08242: Methyltransf_12" amino acids 186 to 280 (95 residues), 53.2 bits, see alignment E=1.6e-17 PF08241: Methyltransf_11" amino acids 186 to 283 (98 residues), 53.7 bits, see alignment E=1.1e-17

Best Hits

KEGG orthology group: K00574, cyclopropane-fatty-acyl-phospholipid synthase [EC: 2.1.1.79] (inferred from 85% identity to sjp:SJA_C1-09030)

Predicted SEED Role

"Cyclopropane-fatty-acyl-phospholipid synthase (EC 2.1.1.79)" (EC 2.1.1.79)

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.79

Use Curated BLAST to search for 2.1.1.79

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (413 amino acids)

>GFF723 Cyclopropane-fatty-acyl-phospholipid synthase (EC 2.1.1.79) (Sphingobium sp. HT1-2)
MKIFDRVLRRLVTKGQLTVFYADGRSVTVGSPDPDLPPLALRFLDKRVPLDIVKDPRLGM
AEAYIDGRVAIEGGGIMEFVSMIRQNNAWENGKSIDAKGPVQRALKTVRRKLWKANHRSR
SKANVAHHYDLSGALYALFLDRDRQYSCAYFPDAENEAGISLEQAQEDKKAHIAAKLYLK
PGMKVLDIGCGWGGMALYLHKHFGVDVTGITLSEEQLKVARQRAIDAGVADHVRFELIDY
RDIEGPFDRIVSVGMFEHVGTADYRVFYNKCRDLLTPDGVMLIHTIGRVDGPGITDTFTT
KYIFPGGYIPALSEMVAGSEGTRLMITDVEVLRIHYGLTIRDWYKRTMAHKADIVALYDE
RFFRLWTFYLAGAATVFEHGSMVNFQVQYVRDRRALPITRDYMIEAETALRGR