Protein Info for GFF7120 in Variovorax sp. SCN45

Annotation: Ortho-halobenzoate 1,2-dioxygenase alpha-ISP protein OhbB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 414 PF00355: Rieske" amino acids 44 to 122 (79 residues), 63.1 bits, see alignment E=1.9e-21 PF00848: Ring_hydroxyl_A" amino acids 188 to 413 (226 residues), 53.1 bits, see alignment E=4.2e-18

Best Hits

KEGG orthology group: None (inferred from 61% identity to bxe:Bxe_B2752)

Predicted SEED Role

"Ortho-halobenzoate 1,2-dioxygenase alpha-ISP protein OhbB" in subsystem Benzoate degradation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (414 amino acids)

>GFF7120 Ortho-halobenzoate 1,2-dioxygenase alpha-ISP protein OhbB (Variovorax sp. SCN45)
MEKTAEQPLVRWPSQGEARVPYRVFAEDEIYRAEQAKIFQGDTWNYVALEVELANAGDFK
STFIGNTPVIVVRESDGEIYAFENRCAHRGALICLENRGTASEFQCVYHAWTYDRKGNLV
GVAFEKGVRGQGGMPDSFCKEHHGPRKLRIATLAGLVFASLSEDVPPLEEYLGDDIVGKI
NRVLFKPLEVIGRFTQVLPNNWKLYAENVRDTYHASLLHLFFTTFNITRLSQAGGVVISE
SGAHHASASVKTPETTPSTDTAYDAHRSSRDGYRLHDASMLASQDEIGDGIHVQILSVFP
GFVLQQIQNCLAVRQIVPQGTDSMDLHWTYIGFADDTPELREMRLKQSNLVGPAGYVSME
DGAIGNFVQRGIAGAHDMESVIELGGFEAKTQDFRATESSIRGFWKAYRAYMGV