Protein Info for GFF71 in Variovorax sp. SCN45

Annotation: Aminoglycoside 3''-nucleotidyltransferase (EC 2.7.7.-) => ANT(3'')-Ia (AadA family)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 269 PF01909: NTP_transf_2" amino acids 21 to 98 (78 residues), 28.4 bits, see alignment E=1.7e-10 PF13427: AadA_C" amino acids 153 to 255 (103 residues), 111.2 bits, see alignment E=2.7e-36

Best Hits

Swiss-Prot: 57% identical to S3AD_ECOLX: Streptomycin 3''-adenylyltransferase (aadA) from Escherichia coli

KEGG orthology group: K00984, streptomycin 3''-adenylyltransferase [EC: 2.7.7.47] (inferred from 65% identity to eum:ECUMN_4816)

Predicted SEED Role

"Streptomycin 3''-O-adenylyltransferase (EC 2.7.7.47) @ Spectinomycin 9-O-adenylyltransferase" (EC 2.7.7.47)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.7.47

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (269 amino acids)

>GFF71 Aminoglycoside 3''-nucleotidyltransferase (EC 2.7.7.-) => ANT(3'')-Ia (AadA family) (Variovorax sp. SCN45)
MTDTLPPAEIAPQLSLARAVLERHLAGTLQALHLFGSAVDGGLKPGSDIDLLVTVTTPPA
EPVRRALMTELLSVSAMPVAGESLRPLEVTVLAREHVVPWRHPARRELQFGEWLREDLLA
GRFEPPMVDHDLAILLTKARRYSIGLLGPDASTLFDPVPAHDFAKALADTVAQWSEPSDW
LGDERNIVLALARVWFSVETGGIASKDVAAAWAIEQLPPEHRPVLATARAAYLGVAQDDL
AARADEVTAFVRHTRATIEHMQRMLPGGR