Protein Info for GFF705 in Sphingobium sp. HT1-2

Annotation: Proline iminopeptidase (EC 3.4.11.5)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 313 TIGR01249: prolyl aminopeptidase" amino acids 9 to 310 (302 residues), 413.3 bits, see alignment E=3e-128 PF12697: Abhydrolase_6" amino acids 38 to 297 (260 residues), 37.9 bits, see alignment E=5e-13 PF00561: Abhydrolase_1" amino acids 38 to 296 (259 residues), 112.9 bits, see alignment E=3.2e-36 PF12146: Hydrolase_4" amino acids 60 to 134 (75 residues), 28.9 bits, see alignment E=1.1e-10

Best Hits

Swiss-Prot: 66% identical to PIP_SERMA: Proline iminopeptidase (pip) from Serratia marcescens

KEGG orthology group: K01259, proline iminopeptidase [EC: 3.4.11.5] (inferred from 90% identity to sjp:SJA_C1-07360)

Predicted SEED Role

"Proline iminopeptidase (EC 3.4.11.5)" in subsystem Proline, 4-hydroxyproline uptake and utilization (EC 3.4.11.5)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.4.11.5

Use Curated BLAST to search for 3.4.11.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (313 amino acids)

>GFF705 Proline iminopeptidase (EC 3.4.11.5) (Sphingobium sp. HT1-2)
MRSLYPPIEPYATGHLDVGDGHSLYWERSGTPGAKPAVFLHGGPGGGISPDHRRLFDPAR
YDVLLFDQRGCGRSTPHANLDANTTWHLVADIERLREMMGVDQWLVFGGSWGSTLALAYA
QAHVSRVTELVLRGIFTIRQSEIDWYYQQGASRIYPDKWERFVAPIPEDERDDMVGAYRR
ILTGDDRAAQVAAAKAWSVWEGETIRLLPDDALSATHEGDDFALAFARIENHYFVHRGWL
EDGQLIREAGKLADIPGVIVQGRYDMACPAETAWALHRAWPQARFEMIEGAGHAFNEPGI
LDALIRATDGFAG