Protein Info for GFF700 in Sphingobium sp. HT1-2

Annotation: Excinuclease ABC subunit B

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 727 TIGR00631: excinuclease ABC subunit B" amino acids 35 to 686 (652 residues), 989 bits, see alignment E=4.7e-302 PF04851: ResIII" amino acids 43 to 168 (126 residues), 49.3 bits, see alignment E=1.7e-16 PF00270: DEAD" amino acids 53 to 115 (63 residues), 26.3 bits, see alignment E=1.7e-09 PF17757: UvrB_inter" amino acids 190 to 278 (89 residues), 102.8 bits, see alignment E=2.7e-33 PF00271: Helicase_C" amino acids 466 to 575 (110 residues), 66.7 bits, see alignment E=6.4e-22 PF12344: UvrB" amino acids 582 to 623 (42 residues), 74.1 bits, see alignment 1.9e-24 PF02151: UVR" amino acids 656 to 688 (33 residues), 33.1 bits, see alignment (E = 1.1e-11)

Best Hits

Swiss-Prot: 78% identical to UVRB_ZYMMO: UvrABC system protein B (uvrB) from Zymomonas mobilis subsp. mobilis (strain ATCC 31821 / ZM4 / CP4)

KEGG orthology group: K03702, excinuclease ABC subunit B (inferred from 95% identity to sch:Sphch_1753)

MetaCyc: 62% identical to UvrABC excision nuclease subunit B (Escherichia coli K-12 substr. MG1655)
3.1.25.-

Predicted SEED Role

"Excinuclease ABC subunit B" in subsystem DNA repair, UvrABC system

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (727 amino acids)

>GFF700 Excinuclease ABC subunit B (Sphingobium sp. HT1-2)
MSIQIRTSLDEPETGQDFVPHRPARPEKSEGGRPFTLVSDYEPSGDQPTAIAELVAAAKQ
GEKDQVLLGVTGSGKTFTMAKTIEALQRPALILAPNKILAAQLYGEFKSFFPDNAVEYFV
SYYDYYQPEAYVARSDTYIEKESSVNESIDRMRHSATRALLERDDVIIVASVSCLYGIGS
VETYSAMTFSMKKGGIEDQREIIRKLVALQYKRNDAGFARGNFRVKGDNLEIFPSHYEDT
AWRISFFGDEIEEIVEFDPLSGRKVASLDYVKVFPNSHHVTPGPTLKQAMEAIRFELTER
LKELEAEGKLLEAQRLEQRTNFDLEMIAATGSCAGIENYSRFLTGRLPGEPPPTLFEYLP
ENAVLFVDESHQTVPQIGAMARGDHRRKITLAEYGFRLPSCIDNRPLRFNEWDAMRPQTV
SVSATPGPWEMEQTGGVFSEQVIRPTGLIDPPVEIKPVEDQVDDLINECRKVAAQGYRTL
VTTLTKRMAEDLTEFMHEAGIKVRYMHSDVETLERIELIRDLRLGVYDVLIGINLLREGL
DIPECGLVAILDADKEGFLRSETSLIQTIGRAARNVDGRVILYADRVTGSMERALNETSR
RREKQQAYNDAHGITPFTVKKNIGDIIAHVASKDQVTVETGLEDRPHLVGHNLRAYIEDL
EKKMRAAAADLEFEEAGRIRDEIRKLEAEELGLPADQQVAAPRGRATEGKPGTRKGRFGK
TTRKFGR