Protein Info for GFF70 in Variovorax sp. SCN45

Annotation: Mannose-1-phosphate guanylyltransferase (EC 2.7.7.13) / Mannose-6-phosphate isomerase (EC 5.3.1.8)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01479: mannose-1-phosphate guanylyltransferase/mannose-6-phosphate isomerase
  • amino acids 7 to 468 (462 residues), 572.7 bits, E=3e-176
• PF00483: NTP_transferase
  • amino acids 9 to 282 (274 residues), 147.2 bits, E=1.7e-46
• PF12804: NTP_transf_3
  • amino acids 10 to 135 (126 residues), 30.4 bits, E=1e-10
• PF22640: ManC_GMP_beta-helix
  • amino acids 296 to 346 (51 residues), 35.7 bits, 1.7e-12
• PF01050: MannoseP_isomer
  • amino acids 352 to 464 (113 residues), 181.3 bits, E=1.5e-57
• PF07883: Cupin_2
  • amino acids 381 to 448 (68 residues), 46 bits, E=8.7e-16

Best Hits

Swiss-Prot: 48% identical to MANC_ECOLI: Mannose-1-phosphate guanylyltransferase (manC) from Escherichia coli (strain K12)

KEGG orthology group: K00971, mannose-1-phosphate guanylyltransferase [EC: 2.7.7.22] K01809, mannose-6-phosphate isomerase [EC: 5.3.1.8] (inferred from 84% identity to vpe:Varpa_2870)

MetaCyc: 48% identical to mannose-1-phosphate guanylyltransferase (Escherichia coli K-12 substr. MG1655)
Mannose-1-phosphate guanylyltransferase. [EC: 2.7.7.13]

Predicted SEED Role

"Mannose-1-phosphate guanylyltransferase (GDP) (EC 2.7.7.22)" in subsystem Alginate metabolism or Mannose Metabolism (EC 2.7.7.22)

MetaCyc Pathways

• colanic acid building blocks biosynthesis (11/11 steps found)
• GDP-mannose biosynthesis (4/4 steps found)
• β-1,4-D-mannosyl-N-acetyl-D-glucosamine degradation (2/3 steps found)
• D-mannose degradation I (1/2 steps found)
• D-mannose degradation II (1/2 steps found)
• 1,5-anhydrofructose degradation (3/5 steps found)
• mannitol degradation II (2/4 steps found)
• superpathway of GDP-mannose-derived O-antigen building blocks biosynthesis (9/14 steps found)
• mannitol biosynthesis (1/3 steps found)
• β-(1,4)-mannan degradation (2/7 steps found)

KEGG Metabolic Maps

• Fructose and mannose metabolism
• Nucleotide sugars metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.7.13 or 2.7.7.22 or 5.3.1.8

Protein Sequence (503 amino acids)

>GFF70 Mannose-1-phosphate guanylyltransferase (EC 2.7.7.13) / Mannose-6-phosphate isomerase (EC 5.3.1.8) (Variovorax sp. SCN45)
MNTNPTIHPVVLCGGSGTRLWPLSRKALPKQFAPLLGGKSLLHLTLERLSGLNHDITCIA
SEDHRFLVKETVEGAGLTGRQILEPVARNTAAAMASAALLAEPGDLLLFAPADHHIPDAA
LFAQTVREGVDAALAGRIVTFGVVPSFPSTAYGYIEAGAVSADGRSRAVARFVEKPTASV
AESLILHGGYYWNAGIFLVRAGTLLAALRTHAPDILRACERAAAAVAVDGSFLRLEQAAF
SACRAESIDYAVLEKHRDIAVVKFEGGWSDVGSWNAVANLQLADGDGNRLSGKASALNSR
NTFIHAPHRPVVALGTDGLIIVDTPDAVLVAGAGCAEQVADVVRKLSREGCAEATDHRRV
VRPWGAYDSVDMGERFQVKRLTVKPGAKLSLQMHHHRAEHWIVVKGTARATCNGEVTLVR
ENESIYLPSGAIHRLENPGKTMLEVIEVQTGGYLGEDDIVRFDDTYGRCEAMRNAEEAAA
APSSPSASAGSGAAAVLHAPVSH