Protein Info for GFF7 in Xanthobacter sp. DMC5

Annotation: 50S ribosomal protein L7/L12

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 124 transmembrane" amino acids 34 to 51 (18 residues), see Phobius details TIGR00855: ribosomal protein bL12" amino acids 1 to 124 (124 residues), 161 bits, see alignment E=8.3e-52 PF16320: Ribosomal_L12_N" amino acids 5 to 50 (46 residues), 64.8 bits, see alignment E=4.6e-22 PF00542: Ribosomal_L12" amino acids 58 to 124 (67 residues), 108.1 bits, see alignment E=2.2e-35

Best Hits

Swiss-Prot: 97% identical to RL7_XANP2: 50S ribosomal protein L7/L12 (rplL) from Xanthobacter autotrophicus (strain ATCC BAA-1158 / Py2)

KEGG orthology group: K02935, large subunit ribosomal protein L7/L12 (inferred from 97% identity to xau:Xaut_3363)

MetaCyc: 56% identical to 50S ribosomal subunit protein L12 (Escherichia coli K-12 substr. MG1655)

Predicted SEED Role

"LSU ribosomal protein L7/L12 (P1/P2)" in subsystem Ribosome LSU bacterial

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (124 amino acids)

>GFF7 50S ribosomal protein L7/L12 (Xanthobacter sp. DMC5)
MADLTKLVDELSSLTVLEAAELAKLLEEKWGVSAAAAVAVAAAPAGAAAAAVEEQTEFTV
MLAAAGDKKIEVIKEVRAITGLGLKEAKDLVEGAPKPVKEGVAKDEAEKLKAQLEKAGAK
VELK