Protein Info for GFF697 in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868

Annotation: ATP-dependent helicase DinG/Rad3

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 714 signal peptide" amino acids 1 to 14 (14 residues), see Phobius details PF00270: DEAD" amino acids 31 to 95 (65 residues), 25.7 bits, see alignment E=1.7e-09 PF06733: DEAD_2" amino acids 196 to 264 (69 residues), 29.5 bits, see alignment E=1.1e-10 PF13307: Helicase_C_2" amino acids 528 to 688 (161 residues), 124.8 bits, see alignment E=7.7e-40

Best Hits

Swiss-Prot: 100% identical to DING_SALTY: ATP-dependent DNA helicase DinG (dinG) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K03722, ATP-dependent DNA helicase DinG [EC: 3.6.4.12] (inferred from 93% identity to cro:ROD_08041)

MetaCyc: 92% identical to ATP-dependent DNA helicase DinG (Escherichia coli K-12 substr. MG1655)
RXN0-4261 [EC: 5.6.2.3]

Predicted SEED Role

"ATP-dependent helicase DinG/Rad3" in subsystem DNA repair, bacterial DinG and relatives

Isozymes

Compare fitness of predicted isozymes for: 3.6.4.12

Use Curated BLAST to search for 3.6.4.12 or 5.6.2.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (714 amino acids)

>GFF697 ATP-dependent helicase DinG/Rad3 (Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868)
MALTAALKAQIAAWYKALQDQIPDFIPRAPQRQMIADVARTLAGEEGRHLAIEAPTGVGK
TLSYLIPGIAIAREEQKTLVVSTANVALQDQIFSKDLPLLRKIIPDLRFTAAFGRGRYVC
PRNLAALASSEPTQQDLLAFLDDELTPNNQEEQKRCARLKGDLDGYKWDGLRDHTDIAID
DDLWRRLSTDKASCLNRNCHYYRECPFFVARREIQEAEVVVANHALVMAAMESEAVLPEP
KHLLLVLDEGHHLPDVARDALEMSAEITASWYRLQLDLFSKLVATCMEQFRPKTTPPLAN
PERLNAHCEEVYELIASLNAILNLYMPAAQEAEHRFAMGELPDEVMEICQRLAKLTETLR
GLAESFLNDLSEKTGSHDIVRLHRVILQMNRALGMFEAQSKLWRLASMAQSSGAPVSKWA
TREIREGQLHVWFHCVGIRVSDQLERLLWRSVPHIIVTSATLRSLNSFSRLQEMSGLKEK
AGDRFVALDSPFNHVEQGKLVIPQMRYEPTIDNEEQHIAEMAAYFREQLESKKHHGMLVL
FASGRAMQRFLEHVADVRLLLLVQGDQPRYRLVELHRKRVESGERSVLVGLQSFAEGLDL
KGELLTQVHIHKIAFPPIDSPVVITEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRS
HACRGEVVIYDKRLLTKNYGQRLLNALPVFPIEQPAVPDVIVKPKAKPARRRRR