Protein Info for GFF6944 in Variovorax sp. SCN45

Annotation: Xylulose-5-phosphate phosphoketolase (EC 4.1.2.9) @ Fructose-6-phosphate phosphoketolase (EC 4.1.2.22)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 790 PF09364: XFP_N" amino acids 14 to 370 (357 residues), 560.9 bits, see alignment E=1.6e-172 PF03894: XFP" amino acids 400 to 576 (177 residues), 287 bits, see alignment E=8.2e-90 PF09363: XFP_C" amino acids 587 to 788 (202 residues), 318.1 bits, see alignment E=3.1e-99

Best Hits

Swiss-Prot: 71% identical to PHK_GLOVI: Probable phosphoketolase (glr0997) from Gloeobacter violaceus (strain ATCC 29082 / PCC 7421)

KEGG orthology group: K01632, fructose-6-phosphate phosphoketolase [EC: 4.1.2.22] (inferred from 70% identity to ava:Ava_0496)

Predicted SEED Role

"Xylulose-5-phosphate phosphoketolase (EC 4.1.2.9) @ Fructose-6-phosphate phosphoketolase (EC 4.1.2.22)" (EC 4.1.2.22, EC 4.1.2.9)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.1.2.22 or 4.1.2.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (790 amino acids)

>GFF6944 Xylulose-5-phosphate phosphoketolase (EC 4.1.2.9) @ Fructose-6-phosphate phosphoketolase (EC 4.1.2.22) (Variovorax sp. SCN45)
MPSSSPESTVPDDFTLLDAWWRAANYLSVGQIYLMDNPLLREKLALPHIKPRLLGHWGTT
PGLNFIYAHMNRAINARELDAIFIAGPGHGGPGVVANTWLEGSYSEVYPEVGRDAAGLRR
LFTRFSFPGGIPSHAAPETPGSIHEGGELGYSLLHAYGAVFDNPSLIACCVVGDGEAETG
ALAASWHSNKFLNPGRDGAVLPVLHLNGYKIAGPTVLARIGEEELTQLLRGYGHEPYFVV
GDKPADMHRQMAAALDTALDGIRAIQANARTHGFRVRPRWPMIVLRSPKGWTGPHYVDGV
QIEGTFRSHQVPLADFSARPQHIELLERWLRSYRPEELFDADGALRPEIAAIAPKGDRRM
SANPHANGGLLLRDLALPPFRAHAVPVATPGATDAEATRVAGGFLREVMRANLPHANFRV
MGPDETASNRLSALFEVTGRTTTAQVWPGDENLSPEGRVMEVLSEHLCQGWLEGYLLTGR
HGFFSCYEAFIHIVDSMFNQHAKWLKVTRGIGWRRPIASLNYLLTSHVWRQDHNGFSHQD
PGFLDHVVSKKAEVVRVYLPPDANTLLSVVDHCLRSRDYVNVIVAGKQPAPQWLDMESAA
RHCTTGLGIWEWASNDEGSAPDVVMACAGDVPTLETMAAVDLLREKLPSLKVRVVNVVDL
MALQSPDAHPHGLPDADFDAIFTTDRPVIFAFHGYPALIHRLIYKRRNHPGFHVHGYCEE
GTTTTPFDMTVLNRLDRFHLAADAIARVPGLCDSAGHVQQHLRDRLLEHRAYITRHGKDM
PEIEDWRWTR