Protein Info for GFF690 in Xanthobacter sp. DMC5

Annotation: Thymidylate kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00041: dTMP kinase
  • amino acids 2 to 200 (199 residues), 161.7 bits, E=9.1e-52
• PF13521: AAA_28
  • amino acids 6 to 154 (149 residues), 35.2 bits, E=1.5e-12
• PF02223: Thymidylate_kin
  • amino acids 8 to 200 (193 residues), 152.7 bits, E=9.4e-49

Best Hits

Swiss-Prot: 61% identical to KTHY_BRUSI: Thymidylate kinase (tmk) from Brucella suis (strain ATCC 23445 / NCTC 10510)

KEGG orthology group: K00943, dTMP kinase [EC: 2.7.4.9] (inferred from 78% identity to xau:Xaut_4325)

MetaCyc: 44% identical to Tmk (Escherichia coli K-12 substr. MG1655)
T(2)-induced deoxynucleotide kinase. [EC: 2.7.4.12, 2.7.4.13, 2.7.4.9]

Predicted SEED Role

"Thymidylate kinase (EC 2.7.4.9)" (EC 2.7.4.9)

MetaCyc Pathways

• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (17/18 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis I (9/9 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (E. coli) (12/14 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis III (8/9 steps found)
• pyrimidine deoxyribonucleotide phosphorylation (4/4 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis IV (6/7 steps found)
• pyrimidine deoxyribonucleotides biosynthesis from CTP (6/8 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis II (5/7 steps found)
• purine deoxyribonucleosides salvage (4/6 steps found)
• superpathway of pyrimidine deoxyribonucleoside salvage (5/9 steps found)

KEGG Metabolic Maps

• Pyrimidine metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.7.4.9

Use Curated BLAST to search for 2.7.4.12 or 2.7.4.13 or 2.7.4.9

Protein Sequence (220 amino acids)

>GFF690 Thymidylate kinase (Xanthobacter sp. DMC5)
MSGRFITLEGGEGTGKSTQARRLAQHLRALGREVVETREPGGTPGAEALRHVILSGAAEP
LGPAAEALLFAAARADHVAGLIAPALAAGKIVVCDRFIDSTRVYQGAVGAVDASLLDSLE
GLSVGAVRPDLTLVLDVPPEIGLARAARRAAGAAADRFEKEGGDYHAAVRAAFLARAAAE
PARCALIDASADADAVAAAIADVVAARLGLGAYEISGAGA