Protein Info for GFF6802 in Variovorax sp. SCN45

Annotation: Uncharacterized MFS-type transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 446 transmembrane" amino acids 29 to 46 (18 residues), see Phobius details amino acids 66 to 85 (20 residues), see Phobius details amino acids 98 to 118 (21 residues), see Phobius details amino acids 124 to 147 (24 residues), see Phobius details amino acids 159 to 180 (22 residues), see Phobius details amino acids 192 to 214 (23 residues), see Phobius details amino acids 260 to 280 (21 residues), see Phobius details amino acids 292 to 313 (22 residues), see Phobius details amino acids 325 to 344 (20 residues), see Phobius details amino acids 350 to 372 (23 residues), see Phobius details amino acids 382 to 405 (24 residues), see Phobius details amino acids 418 to 440 (23 residues), see Phobius details PF07690: MFS_1" amino acids 37 to 401 (365 residues), 170.9 bits, see alignment E=1.9e-54

Best Hits

Swiss-Prot: 46% identical to NICT_PSEPK: Putative metabolite transport protein NicT (nicT) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)

KEGG orthology group: None (inferred from 92% identity to vpe:Varpa_4232)

Predicted SEED Role

"Nitrate/nitrite transporter" in subsystem Nitrate and nitrite ammonification

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (446 amino acids)

>GFF6802 Uncharacterized MFS-type transporter (Variovorax sp. SCN45)
MNTATLGATPAHASPETAARDDAALFRKITWRLMPLLCACYVLNYLDRTNVGYAQLQMKE
QLGFSDAVFGLGAGIFFIGYAVFEIPSNLMLAKIGVRATLLRIMGLWGLASAAMMFATTP
TQFYVLRFLIGVFEAGFAPGVLFYLTLWFPSHRRAQATALFFMAFGAAPIVAGPIAGLTM
TCLDGVLSLRGWQWLFLLEGVPSVLLGLAAFRWLSNSPAQAPWLSAAEKQRVARLLAEDQ
AAHGAAERHTFGAAMRDGRVWLIGFMSFLIILGIYALSFWQPTILKSMGLSVLQIGFYAV
IPAVAGIAANIVVGRRSDRRKERRWHFALSAVVGAAGLALTTLFMHDALAAVLCLALASM
GISAAFTVLWAVPGTFMSRSAAAAGIALISTIGGSAGLVAPMMVGALKTLTGGFTTSLYV
LSGALVLSALLMLVAMPASALGRAPR