Protein Info for PS417_03460 in Pseudomonas simiae WCS417

Annotation: type III secretion protein HrpI

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 697 transmembrane" amino acids 20 to 38 (19 residues), see Phobius details amino acids 44 to 63 (20 residues), see Phobius details amino acids 75 to 95 (21 residues), see Phobius details amino acids 108 to 133 (26 residues), see Phobius details amino acids 202 to 223 (22 residues), see Phobius details amino acids 243 to 265 (23 residues), see Phobius details amino acids 288 to 304 (17 residues), see Phobius details amino acids 308 to 326 (19 residues), see Phobius details TIGR01399: type III secretion protein, HrcV family" amino acids 17 to 684 (668 residues), 837.1 bits, see alignment E=5.5e-256 PF00771: FHIPEP" amino acids 29 to 674 (646 residues), 666.5 bits, see alignment E=2.4e-204

Best Hits

Swiss-Prot: 61% identical to HRPI_ERWAM: Harpin secretion protein HrpI (hrpI) from Erwinia amylovora

KEGG orthology group: K03230, type III secretion protein SctV (inferred from 68% identity to pba:PSEBR_a5388)

Predicted SEED Role

"Flagellar biosynthesis protein FlhA" in subsystem Flagellar motility or Flagellum

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7U1S4 at UniProt or InterPro

Protein Sequence (697 amino acids)

>PS417_03460 type III secretion protein HrpI (Pseudomonas simiae WCS417)
MTALSAINSFLLKVAQRAEVLGAVVVMAIVFIFIVPLPTWLVDILIALNICISCLLIVLA
LYLPGPLAFSSFPSILLLTTMFRLALSIATTRLILLEQDAGHIVEAFGNFVVGGNLAVGM
VIFLILTLVNFLVITKGSERVAEVAARFSLDAMPGKQMSIDSDLRAGLIDGMQARDKREQ
LSRESQLFGAMDGAMKFVKGDAIAGLIIVVVNLLGGFSTGMFQHGLSASESISLYSVLTI
GDGLIAQIPALLISLTAGMIITRVAPDGRRGASNMGAEIARQMTSEPKSWMIASVGMLAF
AVVPGMPTGVFILIALVTGSLGYYLMRQRQRQEQPAAETAEAVRPEENGSEDLRGFDPSR
PYLLQFSPVIRGTPEVADLVHSIRQARNALVANIGLTLPPFEVELDDSLADDEMRFCVHE
VPMVKASVVSHVAVERKALSVEPAHAIAGLVERDEQDWLWLAPDDPLLDDPQLERFTAAS
LIVERMKQAMMLSGPQFLGIQESKSILSWLEHNQPELVQELQRIMPLSRFSAVLQRLASE
GVPLRAVRLIVESLIEYGQHEREPDALADYARIALKAQIYHQYSEADGLHAWLLSPQTEN
ILREALRQTQTGVFFALDDEHSAVLVSLLNQAFPVRPKLKSVMLVAQDLRSPLRTLLLEE
FNHVPVLSFAELGSASKVKVLGRFDLGQDELMRGAVA