Protein Info for GFF680 in Sphingobium sp. HT1-2

Annotation: Ribonucleotide reductase of class Ia (aerobic), alpha subunit (EC 1.17.4.1)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00317: Ribonuc_red_lgN
  • amino acids 49 to 114 (66 residues), 65.8 bits, E=3e-22
• PF02867: Ribonuc_red_lgC
  • amino acids 118 to 437 (320 residues), 255 bits, E=1.5e-79
  • amino acids 446 to 592 (147 residues), 129.8 bits, E=1.3e-41

Best Hits

Swiss-Prot: 44% identical to RIR1_FRG3G: Putative ribonucleoside-diphosphate reductase large subunit (FV3-038R) from Frog virus 3 (isolate Goorha)

KEGG orthology group: K00525, ribonucleoside-diphosphate reductase alpha chain [EC: 1.17.4.1] (inferred from 94% identity to sjp:SJA_C1-32220)

Predicted SEED Role

"Ribonucleotide reductase of class Ia (aerobic), alpha subunit (EC 1.17.4.1)" in subsystem Ribonucleotide reduction (EC 1.17.4.1)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (42/46 steps found)
• superpathway of purine nucleotides de novo biosynthesis I (21/21 steps found)
• superpathway of purine nucleotides de novo biosynthesis II (23/26 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (17/18 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis I (9/9 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (E. coli) (12/14 steps found)
• superpathway of guanosine nucleotides de novo biosynthesis I (6/6 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis III (8/9 steps found)
• superpathway of adenosine nucleotides de novo biosynthesis I (5/5 steps found)
• superpathway of guanosine nucleotides de novo biosynthesis II (7/8 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis IV (6/7 steps found)
• superpathway of adenosine nucleotides de novo biosynthesis II (6/7 steps found)
• superpathway of purine nucleotide salvage (11/14 steps found)
• adenosine deoxyribonucleotides de novo biosynthesis I (2/2 steps found)
• guanosine deoxyribonucleotides de novo biosynthesis I (2/2 steps found)
• pyrimidine deoxyribonucleotides biosynthesis from CTP (6/8 steps found)
• adenosine deoxyribonucleotides de novo biosynthesis II (3/4 steps found)
• guanosine deoxyribonucleotides de novo biosynthesis II (3/4 steps found)

KEGG Metabolic Maps

• Glutathione metabolism
• Purine metabolism
• Pyrimidine metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.17.4.1

Use Curated BLAST to search for 1.17.4.1

Protein Sequence (632 amino acids)

>GFF680 Ribonucleotide reductase of class Ia (aerobic), alpha subunit (EC 1.17.4.1) (Sphingobium sp. HT1-2)
VATIMDERIEDLVDKAEPKAAKGKATESASSKIHERRFHVVTDSSRDALLTEFGKDTLND
RYLLPGESYQDLFARVAAAYSDDADHAQRVYDYISKLWFMPATPVLSNGGTGRGLPISCY
LNSVDDSLEGIVNTWNENVWLASRGGGIGTYWGSVRGIGEPVGLNGKTSGIIPFVRVMDS
LTLAISQGSLRRGSAACYLDVSHPEIEEFLEIRKTSGDFNRKALNLHHGVLLTDEFMEAV
RDGSDFNLRSPKDQSVRGTVNARALFQKLVEVRLATGEPYIVFSDTVNRMMPKHHRDLGL
KVSTSNLCSEITLPTGRDHLGQDRTAVCCLSSMNLETWDEWKDHPTFAEDIMRFLDNVLQ
DYIDRAPPEMARAKYSASRERSVGLGVMGFHSFLQARGIPFEGAMAKSWNLRIFKHINAK
VNEASMMLAQERGPCPDAADQGVMERFSCKMAIAPTASISIICGGTSACIEPIPANIYTH
KTLSGSFAVKNPYLEKLLVEKAKDSSAVWNSILERGGSVQHLDFLTQEEKDVYKTSFEID
QRWLLELAADRTPYIDQAQSLNLFIPADVEKWDLLMLHFRAWELGIKSLYYLRSKSVQRA
GFAGGVEADNTIDAPKFEIGETTDYDECLACQ