Protein Info for GFF6668 in Variovorax sp. SCN45

Annotation: Glutaminyl-tRNA synthetase (EC 6.1.1.18)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 604 PF00749: tRNA-synt_1c" amino acids 57 to 378 (322 residues), 325 bits, see alignment E=6.1e-101 TIGR00440: glutamine--tRNA ligase" amino acids 58 to 604 (547 residues), 639.8 bits, see alignment E=2.1e-196 PF03950: tRNA-synt_1c_C" amino acids 387 to 491 (105 residues), 87.9 bits, see alignment E=6.3e-29 PF20974: tRNA-synt_1c_C2" amino acids 511 to 581 (71 residues), 68.5 bits, see alignment E=1e-22

Best Hits

Swiss-Prot: 62% identical to SYQ_CUPTR: Glutamine--tRNA ligase (glnS) from Cupriavidus taiwanensis (strain DSM 17343 / BCRC 17206 / CIP 107171 / LMG 19424 / R1)

KEGG orthology group: K01886, glutaminyl-tRNA synthetase [EC: 6.1.1.18] (inferred from 94% identity to vpe:Varpa_4160)

Predicted SEED Role

"Glutaminyl-tRNA synthetase (EC 6.1.1.18)" in subsystem tRNA aminoacylation, Glu and Gln (EC 6.1.1.18)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.18

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (604 amino acids)

>GFF6668 Glutaminyl-tRNA synthetase (EC 6.1.1.18) (Variovorax sp. SCN45)
MTSPTDKNGAKTTAAPSNFLRHVIENDLAQGAYAGRKWGGSPGSAAHHAQGMADPAKVRM
RFPPEPNGYLHIGHAKSIWLNFELAKEYGGVCHLRFDDTNPEKEDQEYVDSIRDAVKWLG
YETYLADRPSAPGTLQEHEYFASNYFDFMYEAAEYLIGAGLAYVDEQTADEVRANRGDFN
TPGTDSPFRTRTPEENLARFRAMRDGQVADGAAILRAKIDMASTNINMRDPALYRVRRAH
HHNTGDKWCIYPMYTYAHPIEDALEQITHSICTLEFEDQRPFYDWLLDRLAEGGLVASPH
PRQYEFARLNVTHVMTSKRKLRQLVEEKYVDGWDDPRMPTIAGLRRRGYTPEALRLFCER
SGTTKSGGWIAYAGLEAALRETMDPVAPRAMAVLDPVKLVITNWGELMGGDDSLDDCSAP
VHPHHPDMGKREFKLGREVWIERTDYEEVQPKGFFRLFPGNKVRLKYGHVIECTGGTKDA
DGKLVEVQATLVPDTKSGTPGADAIKVKGNITWVAAADAVQAEVRLYERLFAEANPGGGE
LLDELNKTSLEVCSAFVEPSLAKAEAGVGIQFERHGYFVRDPKGDIEGKPVFNRAAGMRD
SWGK