Protein Info for GFF664 in Sphingobium sp. HT1-2

Annotation: Phosphate:acyl-ACP acyltransferase PlsX (EC 2.3.1.n2)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF02504: FA_synthesis
  • amino acids 1 to 317 (317 residues), 347.3 bits, E=4.1e-108
• TIGR00182: fatty acid/phospholipid synthesis protein PlsX
  • amino acids 1 to 325 (325 residues), 330.6 bits, E=5.6e-103

Best Hits

Swiss-Prot: 78% identical to PLSX_SPHAL: Phosphate acyltransferase (plsX) from Sphingopyxis alaskensis (strain DSM 13593 / LMG 18877 / RB2256)

KEGG orthology group: K03621, glycerol-3-phosphate acyltransferase PlsX [EC: 2.3.1.15] (inferred from 87% identity to sjp:SJA_C1-32380)

MetaCyc: 42% identical to acyl phosphate synthase subunit (Streptococcus pneumoniae R6)
RXN-9590 [EC: 2.3.1.274]

Predicted SEED Role

"Phosphate:acyl-ACP acyltransferase PlsX" in subsystem Glycerolipid and Glycerophospholipid Metabolism in Bacteria

MetaCyc Pathways

• CDP-diacylglycerol biosynthesis I (4/4 steps found)
• CDP-diacylglycerol biosynthesis II (4/4 steps found)
• phosphatidylglycerol biosynthesis I (5/6 steps found)
• phosphatidylglycerol biosynthesis II (5/6 steps found)
• CDP-diacylglycerol biosynthesis III (4/5 steps found)
• superpathway of phospholipid biosynthesis III (E. coli) (9/12 steps found)
• oleate biosynthesis III (cyanobacteria) (2/3 steps found)
• superpathway of cardiolipin biosynthesis (bacteria) (9/13 steps found)
• phosphatidate biosynthesis (yeast) (3/5 steps found)
• palmitoyl ethanolamide biosynthesis (2/6 steps found)
• superpathway of stearidonate biosynthesis (cyanobacteria) (2/6 steps found)
• diacylglycerol and triacylglycerol biosynthesis (2/7 steps found)
• stigma estolide biosynthesis (2/7 steps found)
• anandamide biosynthesis II (2/8 steps found)
• anandamide biosynthesis I (3/12 steps found)
• superpathway of phospholipid biosynthesis II (plants) (9/28 steps found)

KEGG Metabolic Maps

• Glycerolipid metabolism
• Glycerophospholipid metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.3.1.15

Use Curated BLAST to search for 2.3.1.15 or 2.3.1.274

Protein Sequence (341 amino acids)

>GFF664 Phosphate:acyl-ACP acyltransferase PlsX (EC 2.3.1.n2) (Sphingobium sp. HT1-2)
MGGDEGVRVMMAGVALARRRHEGLRFTLFGDETRIKAALDNHPNLRAASEVVHSDTIVEG
TDKPSVAIRKKSSSMSMAINAVKSGDAGAAVSAGNTGALMAMAKLALRTMPGVDRPALAA
MLPTLGDNDVVMLDLGANTECDARNLVQFAVMGAAYARIALDLERPRVRLLNIGTEELKG
TDEIRDAAAVLRAADTLPFSFDGFTEGDKIGRGETDVIVCDGFSGNVALKTAEGTARFVA
DLLRRAFSSSLRSKIGFLISKPAMHLLKHHLDPNNHNGAVFLGLNGVVVKSHGSADAKGV
ANAVHVAARLLEEDITRRIAADMAGVQDLSAVASKLELPVK