Protein Info for PS417_03355 in Pseudomonas simiae WCS417

Annotation: cation transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1045 transmembrane" amino acids 12 to 31 (20 residues), see Phobius details amino acids 346 to 363 (18 residues), see Phobius details amino acids 368 to 387 (20 residues), see Phobius details amino acids 395 to 418 (24 residues), see Phobius details amino acids 452 to 474 (23 residues), see Phobius details amino acids 480 to 507 (28 residues), see Phobius details amino acids 539 to 558 (20 residues), see Phobius details amino acids 881 to 900 (20 residues), see Phobius details amino acids 907 to 926 (20 residues), see Phobius details amino acids 933 to 958 (26 residues), see Phobius details amino acids 979 to 999 (21 residues), see Phobius details amino acids 1010 to 1033 (24 residues), see Phobius details TIGR00914: heavy metal efflux pump, CzcA family" amino acids 1 to 1042 (1042 residues), 1706.5 bits, see alignment E=0 PF00873: ACR_tran" amino acids 6 to 1035 (1030 residues), 1135.9 bits, see alignment E=0

Best Hits

Swiss-Prot: 72% identical to CZCA_ALCSC: Cation efflux system protein CzcA (czcA) from Alcaligenes sp. (strain CT14)

KEGG orthology group: K07239, heavy-metal exporter, HME family (inferred from 74% identity to ajs:Ajs_1849)

MetaCyc: 83% identical to cation efflux system protein (Pseudomonas putida KT2440)
RXN1G01-61; TRANS-RXN0-200; TRANS-RXN0-244

Predicted SEED Role

"Cobalt-zinc-cadmium resistance protein CzcA; Cation efflux system protein CusA"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7U9S7 at UniProt or InterPro

Protein Sequence (1045 amino acids)

>PS417_03355 cation transporter (Pseudomonas simiae WCS417)
MFERLIQFAIEQRIIVLLAVLLMAGVGIASYQKLPIDAVPDITNVQVQINSAAAGFSPLE
TEQRITFPIETAMAGLPGLQQTRSLSRSGLSQVTVIFKDGTDLFFARQLVNERLQVAREQ
LPLGIETAMGPISTGLGEIFLWTVEAKEGARKDDGTPYTPTDLRVIQDWIIKPQLRNVPG
VAEINTIGGFAKEYQIAPDPKRLAAYNLTLNDLVTALERNNANVGAGYIERSGEQLLIRA
PGQLASIEDIANIVITSSDGTPIRVRNVAQVEIGRELRTGAATENGREVVLGTVFMLIGE
NSRSVSQAVAKKLEAINRSLPEGVVAVTVYDRTNLVEKAISTVKKNLFEGALLVVAVLFL
FLGNIRAALITAMVIPLAMLFTFTGMFTNKVSANLMSLGALDFGIIVDGAVVIVENAIRR
LAHAQHRHGRLLTRSERLHEVFAAAKEARRALIFGQLIIMVVYLPIFALTGVAGKMFHPM
AFTVVIALLGAMILSVTFVPAAIALFVTGKVKEEENLVMRSARRVYAPVLDWVMARRPWV
FGLAVLTIVASGVVASRMGSEFIPSLSEGDFAQQALRVPGTSLTQSVQMQQQLEKTLMAQ
VPEIERVFARTGTAEIASDPMPPNISDSYVMLKPKDQWPDPRKSRETLIADIQRASAIVP
GSAYELSQPIQLRFNELISGVRSDVAVKVFGDDMDVLNKTAGEIAETLQKLGGASEVKVE
QTSGLPVLTINIDRDKAARFGLNVGDVQDTLAVAVGGRQAGTLYEGDRRFDMVVRLSDAL
RTDIDGLSRLLIPVPGNASGQLGFIALSQVASLDLVLGPNQISRENGKRLVIVSANVRGR
DIGSFVEEAEAAILAQVKVPAGYWTTWGGQFEQLKEASERLRIVVPVALLLVFGLLFMMF
NNLKDGLLVFTGIPFALTGGIMALWLRDIPLSISAGVGFIALSGVAVLNGLVMIAFIRNL
REEGRSLSMAINEGALTRLRPVLMTALVASLGFIPMALATGTGAEVQRPLATVVIGGIIS
STLLTLLVLPALYQWAHRKDEETPG