Protein Info for GFF66 in Sphingobium sp. HT1-2

Annotation: Cobyric acid synthase (EC 6.3.5.10)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 482 PF13500: AAA_26" amino acids 3 to 229 (227 residues), 63.7 bits, see alignment E=3.4e-21 PF01656: CbiA" amino acids 4 to 236 (233 residues), 79.1 bits, see alignment E=4.4e-26 TIGR00313: cobyric acid synthase CobQ" amino acids 4 to 475 (472 residues), 433.3 bits, see alignment E=6e-134 PF07685: GATase_3" amino acids 253 to 433 (181 residues), 183 bits, see alignment E=7.5e-58

Best Hits

Swiss-Prot: 78% identical to COBQ_SPHWW: Cobyric acid synthase (cobQ) from Sphingomonas wittichii (strain RW1 / DSM 6014 / JCM 10273)

KEGG orthology group: K02232, adenosylcobyric acid synthase [EC: 6.3.5.10] (inferred from 78% identity to swi:Swit_4734)

Predicted SEED Role

"Cobyric acid synthase (EC 6.3.5.10)" (EC 6.3.5.10)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.5.10

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (482 amino acids)

>GFF66 Cobyric acid synthase (EC 6.3.5.10) (Sphingobium sp. HT1-2)
MGAIMLQGTGSDVGKSVLVAGLCRALLRRGYRARPFKPQNMSNNAAVTVDGGEIGRAQAL
QAIACGVEAHTDMNPVLMKPQADRTSQLIVHGQVRGTLGSGNFRSARGQLLGAVLESYER
LKAQCDIVVVEGAGSPAEINLRAGDIANMGFARAANVPVLLVGDIDRGGVIAAIVGTRAI
IDPEDAAMIRGFLINKFRGDPALFEDGYRQIEALSGWRGFGVIPWLEATARLPSEDAVVL
ERATPTETERTIIACPILPRISNFDDLDPFKLDPGVELRMVPPGQPIPAEAALIVLPGSK
ATIADMAAMRAQGWDIDILAHHRRGGAILGICGGYQMLGRHIADPDGIEGPAGTVEGLGL
LDVETRLSVGKRLERVDGVALGAVFAGYEMHIGETTGPDSDRPFTHLGRNADGAISADGR
VMGTYVHGLLASSPLRAALLARLGVDGGVADYDRSVDMALDAIAARLEEHLDIDAIVALA
LG