Protein Info for GFF656 in Xanthobacter sp. DMC5

Annotation: Nicotinate dehydrogenase subunit A

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00111: Fer2
  • amino acids 8 to 60 (53 residues), 26 bits, E=7e-10
• PF01799: Fer2_2
  • amino acids 75 to 147 (73 residues), 96.9 bits, E=6.2e-32

Best Hits

Swiss-Prot: 64% identical to NICA_PSEPK: Nicotinate dehydrogenase subunit A (nicA) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)

KEGG orthology group: None (inferred from 86% identity to xau:Xaut_4210)

MetaCyc: 64% identical to nicotinate hydroxylase small subunit (Pseudomonas putida KT2440)
NICOTINATE-DEHYDROGENASE-RXN [EC: 1.17.2.1]

Predicted SEED Role

"Isoquinoline 1-oxidoreductase alpha subunit (EC 1.3.99.16)" in subsystem N-heterocyclic aromatic compound degradation (EC 1.3.99.16)

MetaCyc Pathways

• nicotinate degradation I (2/6 steps found)
• superpathway of nicotinate degradation (6/18 steps found)

Isozymes

Compare fitness of predicted isozymes for: 1.3.99.16

Use Curated BLAST to search for 1.17.2.1 or 1.3.99.16

Protein Sequence (162 amino acids)

>GFF656 Nicotinate dehydrogenase subunit A (Xanthobacter sp. DMC5)
MSAPILLQVNGETHAFALPRETPLIYVLRNDLGLNGPKFGCGLGECGACTVLMDGRATRS
CVTTLGTAEGRAVTTLEGLATQGTLHPVQAAFIAEQAAQCGYCLNGMIMTTVAFLARNPS
PTEAEARAALRYNLCRCGTHVEILRAVLRAAAGSSESVEVQP