Protein Info for GFF656 in Variovorax sp. SCN45

Annotation: macromolecule metabolism; macromolecule degradation; degradation of proteins, peptides, glycopeptides

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 748 signal peptide" amino acids 1 to 28 (28 residues), see Phobius details PF17900: Peptidase_M1_N" amino acids 95 to 300 (206 residues), 81.5 bits, see alignment E=8.6e-27 PF01433: Peptidase_M1" amino acids 341 to 561 (221 residues), 158.8 bits, see alignment E=1.6e-50

Best Hits

KEGG orthology group: K01256, aminopeptidase N [EC: 3.4.11.2] (inferred from 89% identity to vpe:Varpa_3579)

Predicted SEED Role

No annotation

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.4.11.2

Use Curated BLAST to search for 3.4.11.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (748 amino acids)

>GFF656 macromolecule metabolism; macromolecule degradation; degradation of proteins, peptides, glycopeptides (Variovorax sp. SCN45)
MKKFNTPVVNHLAITAVTLAVLTACGGGGDSGGGASLGGGGVGLGASSGPVVSAPAADPT
HKAPDDGVPLAANDAAAAAVDRSAKPQELPDTVWPSNYKLWFRPDAALKTFVGRGDVEIE
VLKPVDAIVVAAHNLNFAKGRTTLRKVSNPGEAIPLIPTPQTLGDFVQLRLNGGQIAKGK
YLLHMEWDGKIQFSDAEFCPPAEMQRNPFCSAATGIFKVGLSTPEGVSSDAIVTQGETNY
ARQWFPGWDEPAFRHTFEISAEVPGDWKTVSNGAQLSAVKLPDGYQQVSFEKTPSMPMYL
TFFGGGKFDILPDTFKNPLDGSDIPLRWFTPPGRSTWATFPMEWTKVAMDYYYKYTGIPL
PIKKFDTIAANDSYNNKPNTGFGGMENWGAIFEFADAVLTKPGDKPTLYSVTVVTHEIAH
QWFGDLVTLDWWDNVWLNESFARWFERRTTIKFHPEYYSFSDYVLDKHSVIVADLRSTAV
PVQRNLNDAGSFGFISPSIFVYNKGSHVLETVQNYIGEEAMQKGLQIYLKDYGFGNVTPS
RLWTSLEKVSGGKKVADIGDSFIRQTGVPLLTVDAQCAADRTYVNITQESFPNQNQFPAS
TWNIPVTLAYGDAMEQRKTFVMSTGAVQVELPGCTAVLAGPTGQDYYVSNYSAQSWSALL
AKAQSFAANKPLLLNIERDAFRLLQVGRITQAQYDQIKGVVNLPAALLAKTGESQQRMAA
VDVGVEKEEYPHALRFQGNLKLRENEKR