Protein Info for GFF652 in Xanthobacter sp. DMC5

Annotation: Enamidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01979: Amidohydro_1
  • amino acids 280 to 377 (98 residues), 32.6 bits, E=5.4e-12

Best Hits

Swiss-Prot: 46% identical to ENA_EUBBA: Enamidase (Ena) from Eubacterium barkeri

KEGG orthology group: None (inferred from 94% identity to xau:Xaut_4206)

MetaCyc: 46% identical to enamidase subunit (Eubacterium barkeri)
Enamidase. [EC: 3.5.2.18]

Predicted SEED Role

"Adenine deaminase (EC 3.5.4.2)" in subsystem Purine conversions (EC 3.5.4.2)

MetaCyc Pathways

• adenine salvage (3/3 steps found)
• nicotinate degradation III (4/9 steps found)
• superpathway of nicotinate degradation (6/18 steps found)

KEGG Metabolic Maps

• Nicotinate and nicotinamide metabolism
• Purine metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.5.4.2

Use Curated BLAST to search for 3.5.2.18 or 3.5.4.2

Protein Sequence (399 amino acids)

>GFF652 Enamidase (Xanthobacter sp. DMC5)
MAHDAPQGPAGPTKLVIRNIGLLLSGDLAKPILDADTVVALDGRITAIGREKDVDTEGAT
TLIDANGTTLTPGLIDSHVHPVAGDWTPRQSQINWIDSSLHGGVTTMISAGEVHYPGRAR
DVVGVKALAITAQRAFSAFRPSGVKVHAGAPVIENEMVEDDFKELAAAGVKLLGEVGLGG
VKDGPTARKMVGWARKYGIQSTIHTGGPSIPGSGLIDKDVVLEADTDVVGHINGGHTALP
DNQIRCICEGCKRGLEIVHNGNERSALYTLRLARELGELQRVILGTDGPAGSGVQPLGIL
RMVSMLSSLGELPAEEAFCLATGNTARMRALDSGLIEVGRAADFVLMDKAQHSPGTTLLD
SVRLGDLPGVCMTIIDGIVRTQRSRNTPPATRMPVIVKG