Protein Info for GFF651 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: Aspartate carbamoyltransferase (EC 2.1.3.2)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF02729: OTCace_N
  • amino acids 16 to 159 (144 residues), 142.1 bits, E=1.5e-45
• TIGR00670: aspartate carbamoyltransferase
  • amino acids 16 to 314 (299 residues), 302.7 bits, E=1.3e-94
• PF00185: OTCace
  • amino acids 167 to 311 (145 residues), 90.4 bits, E=1.3e-29

Best Hits

Swiss-Prot: 89% identical to PYRB_ACIAC: Aspartate carbamoyltransferase (pyrB) from Acidovorax citrulli (strain AAC00-1)

KEGG orthology group: K00609, aspartate carbamoyltransferase catalytic subunit [EC: 2.1.3.2] (inferred from 89% identity to aav:Aave_0910)

Predicted SEED Role

"Aspartate carbamoyltransferase (EC 2.1.3.2)" in subsystem De Novo Pyrimidine Synthesis (EC 2.1.3.2)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (44/46 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (18/18 steps found)
• superpathway of pyrimidine ribonucleotides de novo biosynthesis (9/9 steps found)
• UMP biosynthesis I (6/6 steps found)
• UMP biosynthesis II (6/6 steps found)
• UMP biosynthesis III (5/6 steps found)

KEGG Metabolic Maps

• Alanine and aspartate metabolism
• Pyrimidine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.1.3.2

Protein Sequence (319 amino acids)

>GFF651 Aspartate carbamoyltransferase (EC 2.1.3.2) (Hydrogenophaga sp. GW460-11-11-14-LB1)
VQRKNPQLNKNGELIHLLSTEGLSRDILTQILDTAANFTSVSNREVKKVPLLRGKSVFNL
FFENSTRTRTTFEIAATRLSADVINLDIARSSTAKGESLLDTIANLSAMAADIFVVRHSE
SGAPYLISQHVAPHVHVVNAGDGRHAHPTQGLLDMYTIRHYKKDFTQLSVAIVGDVLHSR
VARSDIHALTTLGCPDVRVVGPRTLVPSDLSHMGVRVCHTLEEGIKDCDVVITLRLQNER
MSGALLPSSQEYFKSFGLTPERLRWAKDDAIVMHPGPINRGVEIDSAVVDGPQSVILPQV
TYGIAVRMAVMGIVAGNEA