Protein Info for GFF650 in Sphingobium sp. HT1-2

Annotation: Phytochrome, two-component sensor histidine kinase (EC 2.7.3.-)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 854 transmembrane" amino acids 612 to 625 (14 residues), see Phobius details PF08446: PAS_2" amino acids 19 to 126 (108 residues), 63.6 bits, see alignment E=6.8e-21 PF01590: GAF" amino acids 152 to 309 (158 residues), 49.8 bits, see alignment E=1.3e-16 PF00360: PHY" amino acids 328 to 506 (179 residues), 160.7 bits, see alignment E=6.5e-51 PF07568: HisKA_2" amino acids 528 to 576 (49 residues), 23.9 bits, see alignment 8.9e-09 PF07536: HWE_HK" amino acids 528 to 610 (83 residues), 77.1 bits, see alignment E=3.5e-25

Best Hits

KEGG orthology group: None (inferred from 86% identity to sjp:SJA_C1-32510)

Predicted SEED Role

"Phytochrome, two-component sensor histidine kinase (EC 2.7.3.-)" in subsystem Oxidative stress or Oxygen and light sensor PpaA-PpsR (EC 2.7.3.-)

Isozymes

Compare fitness of predicted isozymes for: 2.7.3.-

Use Curated BLAST to search for 2.7.3.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (854 amino acids)

>GFF650 Phytochrome, two-component sensor histidine kinase (EC 2.7.3.-) (Sphingobium sp. HT1-2)
VTDVTSPEAQGFTVDINNCDREPIHVLGTVQPFGFLVALTADWLVSRVSTNSADHIGLAP
EQMLGQPISALFTADAIHTLRNRITLLRGPDSVERVFSLPLVEGGAPFDVAVHFSGQLVV
IEAEPAVPDEMEASSMVRSMVARLAQADGMTAFLRDGARQVRALTGFDRVMVYRFADNGD
GEVVAEALRPGIDSFFGLHYPASDIPAQARALYMRNIFRVIADINAPPVPVIPTLDPAGA
ALDMSLCVTRAVSPIHIEYLRNMGVGASLSISIIVDGKLWGLFACHHYAPRLPSFAQRSA
AELFGQIFSMMLEGRERAETASYEGKARQVADRLLSAVAQDHDLMSNARWLGDIIFDTIP
ADGVGIYIDGQMTLSGLTPDEPSFAAIVSMLNRTAASQVYTTDSIARIMPEALAHADRAA
GLLAIPISRRPRDYVVLFRAEQLRSVRWAGKQDKHIEYGPNGPRLTPRKSFEEWSELVKG
TAVAFMPAELRVAEALRTALLEVILRLSDSADEERQRAHEKQELLIAELNHRVRNILALI
RGLISQTRDSAMSVDQFIGTLESRVHALARAHDQITADRWAPARLYDLIEVEAGAYLGDR
RDRVKLTGPNVLLTPAAFTVLALVIHELLTNAAKYGALSDNGSVAIDWHVDAEGSLLLHW
LESGGPAVVAPSRRGFGSTVIERSIPYDLDGRAEVNYRLAGLEARFCIPSAHVASILPDV
AGTDRAKPAPAPSPHLLKGRQILLVEDNMIIAMDGEDALRDLGAQVVTAASIGRAHEAIT
LHPVDLAVLDFNLGNETSLPIADMLAERGVPFLFATGYGDGLDLPDRFSDVILLKKPYSG
ATLAQAIAPMLARG