Protein Info for GFF6464 in Variovorax sp. SCN45

Annotation: Electron transfer flavoprotein-ubiquinone oxidoreductase (EC 1.5.5.1)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 565 PF01266: DAO" amino acids 19 to 60 (42 residues), 28.1 bits, see alignment 3.8e-10 PF13450: NAD_binding_8" amino acids 22 to 66 (45 residues), 28.2 bits, see alignment 4.8e-10 PF21162: ETFQO_UQ-bd" amino acids 221 to 322 (102 residues), 129.8 bits, see alignment E=1.2e-41 PF05187: Fer4_ETF_QO" amino acids 461 to 562 (102 residues), 172.5 bits, see alignment E=5.5e-55

Best Hits

Swiss-Prot: 56% identical to ETFD_MOUSE: Electron transfer flavoprotein-ubiquinone oxidoreductase, mitochondrial (Etfdh) from Mus musculus

KEGG orthology group: K00311, electron-transferring-flavoprotein dehydrogenase [EC: 1.5.5.1] (inferred from 97% identity to vpe:Varpa_2339)

Predicted SEED Role

"Electron transfer flavoprotein-ubiquinone oxidoreductase (EC 1.5.5.1)" in subsystem Acetyl-CoA fermentation to Butyrate or Anaerobic respiratory reductases (EC 1.5.5.1)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.5.5.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (565 amino acids)

>GFF6464 Electron transfer flavoprotein-ubiquinone oxidoreductase (EC 1.5.5.1) (Variovorax sp. SCN45)
MTHDEILAQFGPREAMEYDVVVVGGGPAGLSTAIRLKQLAAQHEKEISVVVLEKGSEPGA
HILSGAIMDPRALNELLPDWKELGAPLNQPVTDDAMVFLGEKGSLRTPNAFLPACFQNHG
NYIISLGAFTKWLAQQAENLGVEIFPGFPAAEVLYNEDGSVRGVATGNMGIGKDGEPTEN
FQLGMELLGKYTVFAEGARGHLGRQLIAKYKLDAGKDPQTYGLGVKEVWEIDPKRHQPGF
VLHTAGWPMKSDTYGGAFLYHMEDNKVTMGFITGLDYSNPYLSPFEEMQRWKLHPNIRWY
LEGDEAKGIKPAKRIGYGARAITAGGLMSLPKTVFPGGALVGCEAGYLNVSRIKGSHAAI
KTGMLCAEAAFEAVQAGRQHDELTAYPEAFEKSWLHTELKKARNFKAWFKKGLGVATLMN
GIEQWLLKGNIPWTLRRNKADHLYLKPASECKPIVYPKPDGKLTFDRLSSVFISNTNHEE
QQPAHLTLKDPTVPVNINLEKFAGPESRYCPAGVYEFVGTDDGKQRLQINAQNCVHCKTC
DIKDPTQNIVWVTPEGGGGPNYVGM