Protein Info for GFF6460 in Variovorax sp. SCN45

Annotation: Sensor protein BasS/PmrB (activates BasR/PmrA)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 451 transmembrane" amino acids 21 to 41 (21 residues), see Phobius details amino acids 162 to 184 (23 residues), see Phobius details PF08521: 2CSK_N" amino acids 29 to 160 (132 residues), 29.8 bits, see alignment E=8.7e-11 PF00512: HisKA" amino acids 241 to 301 (61 residues), 37.4 bits, see alignment E=3.2e-13 PF02518: HATPase_c" amino acids 346 to 446 (101 residues), 51.8 bits, see alignment E=1.6e-17

Best Hits

KEGG orthology group: K02484, two-component system, OmpR family, sensor kinase [EC: 2.7.13.3] (inferred from 89% identity to vpe:Varpa_2344)

Predicted SEED Role

"Sensor protein basS/pmrB (EC 2.7.3.-)" in subsystem Lipid A modifications or Orphan regulatory proteins (EC 2.7.3.-)

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3, 2.7.3.-

Use Curated BLAST to search for 2.7.13.3 or 2.7.3.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (451 amino acids)

>GFF6460 Sensor protein BasS/PmrB (activates BasR/PmrA) (Variovorax sp. SCN45)
MKFGSKPGEGRDSLAGRLTRTLIVWVGGVWLMCVLAVVWYVDREINHNFDNELVEVSHRM
FDMAVQELDKLQHTSGTAPTEPLIAPKQLFSEDAVMYQVVDTHEHVLLRSAETPAGTFDV
PLAAGFANNQTWRIYTVRHPTLGLYFQVADPLDERRRALNRTLFGLIIPLGAVLPLLALV
LRNVARAELRVLQQLAGEIAKRNGADLRPITLPGLPQELRSVGDHVNRLLERLSQSLDVE
RALAANAAHELRTPLAAARLRLQTALEHDLRRNDVQAALDALQMLSHRAEKLLQLSRAES
GASLARARVDLVQLVGTVAQEMWQDPQVNERLVLKVPDSAAPVALGDVDALAIALRNLVE
NALRYGGERVTVEVMAPCTLVVRDFGAGVGAEQLQTLRQRHVRHASDRAGYGLGLSIVGT
IVEKHNARLELASPPPGAAHGFEARIVLQPA