Protein Info for GFF642 in Xanthobacter sp. DMC5

Annotation: K(+)-insensitive pyrophosphate-energized proton pump

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 713 signal peptide" amino acids 1 to 19 (19 residues), see Phobius details transmembrane" amino acids 57 to 93 (37 residues), see Phobius details amino acids 125 to 152 (28 residues), see Phobius details amino acids 159 to 179 (21 residues), see Phobius details amino acids 234 to 254 (21 residues), see Phobius details amino acids 260 to 282 (23 residues), see Phobius details amino acids 294 to 317 (24 residues), see Phobius details amino acids 328 to 352 (25 residues), see Phobius details amino acids 388 to 425 (38 residues), see Phobius details amino acids 470 to 487 (18 residues), see Phobius details amino acids 494 to 510 (17 residues), see Phobius details amino acids 517 to 544 (28 residues), see Phobius details amino acids 586 to 608 (23 residues), see Phobius details amino acids 617 to 640 (24 residues), see Phobius details amino acids 696 to 710 (15 residues), see Phobius details TIGR01104: V-type H(+)-translocating pyrophosphatase" amino acids 5 to 712 (708 residues), 884.8 bits, see alignment E=2.5e-270 PF03030: H_PPase" amino acids 12 to 707 (696 residues), 849.6 bits, see alignment E=9.6e-260

Best Hits

Swiss-Prot: 80% identical to HPPA_RHOPA: K(+)-insensitive pyrophosphate-energized proton pump (hppA) from Rhodopseudomonas palustris (strain ATCC BAA-98 / CGA009)

KEGG orthology group: K01507, inorganic pyrophosphatase [EC: 3.6.1.1] (inferred from 94% identity to xau:Xaut_4197)

Predicted SEED Role

"Pyrophosphate-energized proton pump (EC 3.6.1.1)" in subsystem Phosphate metabolism (EC 3.6.1.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.6.1.1

Use Curated BLAST to search for 3.6.1.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (713 amino acids)

>GFF642 K(+)-insensitive pyrophosphate-energized proton pump (Xanthobacter sp. DMC5)
MLALVLIIVCGLLAVAYGVWTIKGLMEADPGTARMQEIASAIAEGAQAYLRRQYATIGVV
GTVIFVLVGVFLGWLVAIGFALGAILSGLAGFIGMNVSVRANVRTAQAATLSLAGGLDLA
FKAGAVTGLLVAGLALLGVSVYFLVLTGFLGFAPNSRTVVDALVALGFGASLISIFARLG
GGIFTKGADVGGDLVGKVEAGIPEDDPRNPATIADNVGDNVGDCAGMAADLFETYAVTVV
ATMVLAAIFFAANPATLATMIVYPLAIGGACILTSIAGTYFVRLGANSSIMGALYKGLMA
TAGFSVIALAVVTWLLPGFGPIQGAGYTGTALFLSGLVGLAVTGAIVVITEYYTGTGYRP
VVSIAQASVTGHGTNIIQGLAVSLESTALPTLVIIAGILCAYGLAGLFGIAIAATTMLGV
AGMIVALDAFGPVTDNAGGIAEMAGLPKEVRQSTDALDAVGNTTKAVTKGYAIGSAGLGA
LVLFAAYNQDLKFFIAQAAPGSYFAGVAPNFSLENPYVVVGLLFGGLLPYLFGAMGMTAV
GRAAGAIVEEVRRQFREKPGIMAGTEKPDYSRAVDMLTKAAIKEMIIPSLLPVLSPIVVY
FVIYFVAGGGVAGKSAAFSAVGAMLLGVIVTGLFVAISMTSGGGAWDNAKKSFEDGFVDK
DGVRHMKGSEAHKASVTGDTVGDPYKDTAGPAVNPMIKITNIVALLLLAVLGH