Protein Info for GFF6408 in Variovorax sp. SCN45

Annotation: Urocanate hydratase (EC 4.2.1.49)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 575 TIGR01228: urocanate hydratase" amino acids 24 to 573 (550 residues), 951.4 bits, see alignment E=7.2e-291 PF17391: Urocanase_N" amino acids 25 to 151 (127 residues), 204.8 bits, see alignment E=5e-65 PF01175: Urocanase" amino acids 154 to 368 (215 residues), 275.8 bits, see alignment E=3.6e-86 PF17392: Urocanase_C" amino acids 371 to 565 (195 residues), 310.3 bits, see alignment E=6.5e-97

Best Hits

Swiss-Prot: 77% identical to HUTU_TRIRP: Urocanate hydratase from Trifolium repens

KEGG orthology group: K01712, urocanate hydratase [EC: 4.2.1.49] (inferred from 97% identity to vpe:Varpa_0184)

Predicted SEED Role

"Urocanate hydratase (EC 4.2.1.49)" in subsystem Histidine Degradation (EC 4.2.1.49)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.2.1.49

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (575 amino acids)

>GFF6408 Urocanate hydratase (EC 4.2.1.49) (Variovorax sp. SCN45)
VNDHYKFALNNPDAADPRHDPTRVIRAPRGSELNCKSWLTEAPFRMLQNNLDAEVAERPQ
DLVVYGGIGRAARNWACYDQILASLKELNDDETLLIQSGKPVGVFKTHENAPRVLLANSN
LVPKWANWEHFNELDRQGLFMYGQMTAGSWIYIGSQGIVQGTFETFVEAGRQHYNNSLAG
KWILTAGLGGMGGAQPLAATLAGAVSLNIECQQSSIDFRLRTRYVDKQARDIDHAFELIK
QHCDAKEAVSIALLGNAADILPELVKRAKAGGLKPDLVTDQTSAHDLINGYLPSGWSVPQ
WQAAMKDASQHDALKKAAAKSCAVHVQAMLDFQAMGIPTVDYGNNIRQVAFDEGVKNAFD
FPGFVPAYIRPLFCEGKGPFRWVALSGDPEDIYKTDAKIKELFPENKHTHRWLDMARERI
SFQGLPARICWLGLGERHIAGLAFNEMVKNGELKAPIVIGRDHLDTGSVASPNRETEAMK
DGTDAVSDWPLLNALLNTAGGATWVSLHHGGGVGMGYSQHSGVVIVCDGTDAAAKRIERV
LWNDPATGVMRHADAGYDIAVATAKKQGLKLPMVK