Protein Info for GFF6329 in Variovorax sp. SCN45

Annotation: Leucyl-tRNA synthetase (EC 6.1.1.4)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 876 TIGR00396: leucine--tRNA ligase" amino acids 5 to 876 (872 residues), 1032.2 bits, see alignment E=0 PF00133: tRNA-synt_1" amino acids 15 to 587 (573 residues), 125.2 bits, see alignment E=5.9e-40 PF09334: tRNA-synt_1g" amino acids 38 to 171 (134 residues), 66.1 bits, see alignment E=5.2e-22 PF13603: tRNA-synt_1_2" amino acids 222 to 415 (194 residues), 216.4 bits, see alignment E=4.8e-68 PF08264: Anticodon_1" amino acids 714 to 840 (127 residues), 55.3 bits, see alignment E=1.4e-18

Best Hits

Swiss-Prot: 79% identical to SYL_ACIAC: Leucine--tRNA ligase (leuS) from Acidovorax citrulli (strain AAC00-1)

KEGG orthology group: K01869, leucyl-tRNA synthetase [EC: 6.1.1.4] (inferred from 79% identity to aav:Aave_4548)

Predicted SEED Role

"Leucyl-tRNA synthetase (EC 6.1.1.4)" (EC 6.1.1.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (876 amino acids)

>GFF6329 Leucyl-tRNA synthetase (EC 6.1.1.4) (Variovorax sp. SCN45)
MNPSYKPSDVESAAQAQWSAADAYRVTEDASRKKYYACSMLPYPSGKLHMGHVRNYTIND
MLTRYLRMSGYNVLMPMGWDAFGLPAENAALKNGVPPAKWTYENIAYMKGQLQAMGLAID
WSREIATCDPSYYKWNQWLFLKMLEKGIAYRKTQVVNWDPVDQTVLANEQVIDGKGWRTG
ATVERREIPGYYLKISDYAEELLEHTQHKLPGWPERVKLMQENWIGKSEGLRFAFTHDIK
DASGKPIQDGRMYVFTTRADTIMGVTFCAVAPEHPLAAHAATLDPKVAAFIEECKNGGTT
EAELATQEKKGVPTGLTVTHPITDEQVPVWVGNYVLIGYGDGAVMGVPAHDERDFAFANK
YGIEIIQVVLVDDEPHFDYHKWQDWYGDKQRGVTINSDNFSGMTYKEAVAAVAHALGQKG
LGELQTTWRLRDWGVSRQRYWGTPIPIIHCEEHGAVPVPEKDLPVVLPTDCVPDGSGNPL
HKHEGFHAGVVCPVCGKPARRETDTMDTFVDSSWYFMRYCDPKNDEAMVAGGADYWMPMD
QYIGGIEHAILHLLYARFWTKVMRDLGLLKADEPFSKLLTQGMVLNHIFYKRNEKGGKDY
FPPTEVTTVLDAQGRIVGGTTADGTKVEYGGVGKMGKSERNGVDPQDLIEKYGADTARLY
TMFTAPPEATLEWNDAAVEGSYRFLRRVWNFGAAQADVAPVAAAGQAFGKGAQALRREVH
TVLRQVDYDYQRMQYNTVVSGAMKLLNALEGFKPDGSAGDAAAVREGFGILLRCLYPATP
HIAQQLWNELGYDKDLGGLLDAPWPTVDVGALVQDEIELMLQVNGKLRGKLSVPAGASKD
EIEKLALACDDFVAFAEGAQPKRVIVVPGRLVNVVL