Protein Info for GFF626 in Xanthobacter sp. DMC5

Annotation: Bifunctional protein GlmU

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01128: IspD
  • amino acids 7 to 133 (127 residues), 43.6 bits, E=7.5e-15
• PF12804: NTP_transf_3
  • amino acids 8 to 141 (134 residues), 77.7 bits, E=2.9e-25
• TIGR01173: UDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase
  • amino acids 8 to 438 (431 residues), 483.3 bits, E=3.6e-149
• PF00483: NTP_transferase
  • amino acids 8 to 206 (199 residues), 43.6 bits, E=7.2e-15
• PF02348: CTP_transf_3
  • amino acids 22 to 127 (106 residues), 25.4 bits, E=2.9e-09
• PF00132: Hexapep
  • amino acids 268 to 302 (35 residues), 32.1 bits, 1.6e-11
  • amino acids 381 to 414 (34 residues), 28.8 bits, 1.8e-10

Best Hits

Swiss-Prot: 89% identical to GLMU_XANP2: Bifunctional protein GlmU (glmU) from Xanthobacter autotrophicus (strain ATCC BAA-1158 / Py2)

KEGG orthology group: K04042, bifunctional UDP-N-acetylglucosamine pyrophosphorylase / Glucosamine-1-phosphate N-acetyltransferase [EC: 2.3.1.157 2.7.7.23] (inferred from 89% identity to xau:Xaut_4419)

Predicted SEED Role

"N-acetylglucosamine-1-phosphate uridyltransferase (EC 2.7.7.23) / Glucosamine-1-phosphate N-acetyltransferase (EC 2.3.1.157)" in subsystem Peptidoglycan Biosynthesis or Sialic Acid Metabolism or UDP-N-acetylmuramate from Fructose-6-phosphate Biosynthesis (EC 2.3.1.157, EC 2.7.7.23)

MetaCyc Pathways

• O-antigen building blocks biosynthesis (E. coli) (10/11 steps found)
• UDP-N-acetyl-D-glucosamine biosynthesis I (5/5 steps found)
• UDP-N-acetyl-D-glucosamine biosynthesis II (4/6 steps found)
• UDP-N-acetyl-D-galactosamine biosynthesis II (4/7 steps found)
• chitin biosynthesis (5/9 steps found)
• peptidoglycan recycling I (7/14 steps found)
• superpathway of UDP-N-acetylglucosamine-derived O-antigen building blocks biosynthesis (7/24 steps found)
• superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis (13/33 steps found)

KEGG Metabolic Maps

• Aminosugars metabolism
• Nucleotide sugars metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.3.1.157 or 2.7.7.23

Protein Sequence (450 amino acids)

>GFF626 Bifunctional protein GlmU (Xanthobacter sp. DMC5)
MSDRSLLVVVLAAGEGTRMKSRLPKVLHKVAGRTMLHHVLAATRAAGATRTAVVVGPGRE
DVAAEATRIVPDAQVFVQTERLGTAHAVLAARAALEQGADDVLVLYADTPLVRPETLARL
RAPLAEGAAVAALGFEPEDPTGYGRLVTSGDELVAIREEKDATAPEKAVRFCNAGLMALS
GKDALSILDRIGNANSKGEYYLTDAVEIARADGRSAVAAKADVDEVAGVNSRIQLAEAEA
ILQKRLRLSAMAEGVTLIAPETVFFSADTVLGRDVIVEPHVVFGPGVTVGDDVVIHAFCH
LEGAQLAPGVSIGPYARLRPGTRLDEGVRIGNFVETKAAHIESGAKVNHLSYVGDAHVGA
GANLGAGTITCNYDGFFKYRTEIGAGAFIGVNSALVAPVTIGAGAYVGTGAVITSDVPED
ALAIARSRQVVKEGWAKDFRAARADVKKKG