Protein Info for GFF6226 in Variovorax sp. SCN45

Annotation: ABC transporter, substrate-binding protein (cluster 12, methionine/phosphonates)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 326 signal peptide" amino acids 1 to 19 (19 residues), see Phobius details TIGR01098: phosphate/phosphite/phosphonate ABC transporter, periplasmic binding protein" amino acids 13 to 243 (231 residues), 153.5 bits, see alignment E=3.8e-49 PF12974: Phosphonate-bd" amino acids 25 to 276 (252 residues), 182.5 bits, see alignment E=4.6e-58

Best Hits

KEGG orthology group: None (inferred from 86% identity to vap:Vapar_0512)

Predicted SEED Role

"Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1)" in subsystem ABC transporter alkylphosphonate (TC 3.A.1.9.1) (TC 3.A.1.9.1)

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (326 amino acids)

>GFF6226 ABC transporter, substrate-binding protein (cluster 12, methionine/phosphonates) (Variovorax sp. SCN45)
MALLASLVPLLACAQGTAREPPMRFGILPLGGAFESRSDWDPLLAELSRAIDRPVSVLSV
NSYEALEQAIQRDEVDMAFLSGKMALDAVTQRRMKVVAQVVRHDGLPGYRALLLSRKAPP
FNSLKDLLAQPERWRLARGEKQSVSGFIVPQLQLFLPNHIAMETRFLSEIVGTHQATALA
VANSEADVATNNTADFERFKQRFPAEADRLQVLWQSDLIPHAQIVMRREYPPELRKRVQV
FLTSYGRAKGPRGDTERLVLKSLHDLAGFVPADNSSLQPAAKLAWQLARQNAMTAQWVND
AAREARLQRIDGGYAEQVGVLKDVSP