Protein Info for GFF62 in Xanthobacter sp. DMC5

Annotation: Cytochrome b/c1

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 424 transmembrane" amino acids 21 to 36 (16 residues), see Phobius details amino acids 43 to 70 (28 residues), see Phobius details amino acids 91 to 112 (22 residues), see Phobius details amino acids 126 to 148 (23 residues), see Phobius details amino acids 155 to 180 (26 residues), see Phobius details amino acids 195 to 215 (21 residues), see Phobius details amino acids 247 to 265 (19 residues), see Phobius details amino acids 306 to 325 (20 residues), see Phobius details amino acids 337 to 357 (21 residues), see Phobius details amino acids 367 to 389 (23 residues), see Phobius details PF00033: Cytochrome_B" amino acids 33 to 218 (186 residues), 252 bits, see alignment E=5.9e-79 PF13631: Cytochrom_B_N_2" amino acids 102 to 268 (167 residues), 158 bits, see alignment E=3.4e-50 PF00032: Cytochrom_B_C" amino acids 276 to 377 (102 residues), 148.9 bits, see alignment E=7.4e-48

Best Hits

Swiss-Prot: 69% identical to CYB_BLAVI: Cytochrome b (petB) from Blastochloris viridis

KEGG orthology group: K00412, ubiquinol-cytochrome c reductase cytochrome b subunit [EC: 1.10.2.2] (inferred from 94% identity to xau:Xaut_2958)

MetaCyc: 58% identical to cytochrome bc1 complex cytochrome b subunit (Arabidopsis thaliana col)

Predicted SEED Role

"Ubiquinol--cytochrome c reductase, cytochrome B subunit (EC 1.10.2.2)" in subsystem Ubiquinone Menaquinone-cytochrome c reductase complexes (EC 1.10.2.2)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.10.2.2

Use Curated BLAST to search for 1.10.2.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (424 amino acids)

>GFF62 Cytochrome b/c1 (Xanthobacter sp. DMC5)
MEGHSTYQPKGKVAQWFESRLPIVGLIHSSAIAYPVPKNLNYMWTFGAILSFMLVCQIVS
GVVLAMHYVAYAPVSFARVEHIMRDVNYGWLIRYIHANGASMFFIAVYIHIFRGMYYGSY
KAPREVLWILGVIIYLLMMATAFMGYVLPWGQMSFWGATVITNLFSAIPWVGTTIVEWLW
GGYSVGDPTLNRFFSLHYLLPFMIAGVVGLHIWALHHVGQNNPDGVDIKNVAKDSVPFTP
YATIKDIFAMVVFLIFFSWFIFYIPNYLGHSDNYIPANPLSTPAHIVPEWYFLPFYAILR
SIPDKLGGVLAMFAAIAVLAFLPWLDTSKVRSAKYRPLFRQFFWVFAVVAVLLGYMGSQP
AEGGALIISRILTAYYFIHFLVILPLLGLFERPKPVPASIADAVLAKAKGGAVVVAGAAG
PETK