Protein Info for GFF615 in Variovorax sp. SCN45

Annotation: beta-glucosidase (EC 3.2.1.21)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 761 signal peptide" amino acids 1 to 33 (33 residues), see Phobius details PF00933: Glyco_hydro_3" amino acids 54 to 367 (314 residues), 229.7 bits, see alignment E=9e-72 PF01915: Glyco_hydro_3_C" amino acids 407 to 642 (236 residues), 185.8 bits, see alignment E=1.4e-58 PF14310: Fn3-like" amino acids 679 to 747 (69 residues), 81.7 bits, see alignment E=5e-27

Best Hits

KEGG orthology group: K05349, beta-glucosidase [EC: 3.2.1.21] (inferred from 85% identity to vpe:Varpa_3557)

Predicted SEED Role

"Beta-glucosidase (EC 3.2.1.21)" in subsystem Beta-Glucoside Metabolism or Fructooligosaccharides(FOS) and Raffinose Utilization (EC 3.2.1.21)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.2.1.21

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (761 amino acids)

>GFF615 beta-glucosidase (EC 3.2.1.21) (Variovorax sp. SCN45)
MHVRFPRPAALALAPACAFLLSLALSLPSASAAPDAADADARIEALIARMTPQEKAGQLS
LYGPADIDTPNNPQAGWRNAQQETADVRAGRLTGLFNNAGLEGKRRLQQIAVKESRLGIP
LIFGADVIHGFRTMFPMPLAEASSWEPALAERTARAAAVEATADGFRWTFAPMVDIARDA
RWGRGLEGAGEDVLLSKRFAAARVRGFQGADLSRPDAMLATPKHFAAYGAAEGGVDYNVS
DISERTLREVYLPPFRAAIDAGALSVMSAFNEIGGIPSTANGDLLTGVLRDEWKFKGFVV
SDYTADEELVEHGYAANPREAAKQAFLAGTDVSMQSGLYMRYLPDLVASGEVPMARLDEA
VRRVLHVKQKLGLFDDPMHGLDGPPASQRAENPEFIALARESARRSIVMLKNERMLLPLP
KAGTKIALIGPFAEGTVDLMGAWSLFPGQSAPVGIDQGLRAALGANSSVTVVRGSGIGAS
LPGGIDAAVAAARDADVVVLAIGESEKMSGEARSRSDIGLPQAQQDLAEAVAATGKPVVV
LLSNGRAMALKGAVRNARAILVTWFLGVQTGNAIADVLFGDFNPSGRLPVSFPQTAGQAP
YYYAHKRTGRPSPESAPGMSFKARYLDATHEALYPFGFGIGYAPVRYDSVELSQDRIAMS
DTLRVRATVTNTGQRDAEEVVQLYVAQRAASVTRPVRELKDFRKLSIAPGASVQVEFTLT
KDDLQFIGRDMKPTVEPGEFDLWVAPSAAEGIRKRFVLTRD