Protein Info for PS417_03085 in Pseudomonas simiae WCS417

Annotation: type II secretion system protein F

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 295 signal peptide" amino acids 1 to 20 (20 residues), see Phobius details transmembrane" amino acids 108 to 129 (22 residues), see Phobius details amino acids 266 to 289 (24 residues), see Phobius details PF00482: T2SSF" amino acids 152 to 280 (129 residues), 63.2 bits, see alignment E=1.2e-21

Best Hits

KEGG orthology group: K12511, tight adherence protein C (inferred from 92% identity to pfs:PFLU0643)

Predicted SEED Role

"Type II/IV secretion system protein TadC, associated with Flp pilus assembly" in subsystem Widespread colonization island

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7U1M3 at UniProt or InterPro

Protein Sequence (295 amino acids)

>PS417_03085 type II secretion system protein F (Pseudomonas simiae WCS417)
MALLACALLLLAAAVLVGGQLLENRRRERRVAERLQGRMAGEDRLGSLMRQLGGSALAQR
SVSLDNETQTLLNRVGWRKASQRSLFAAFQIGTPIVSLGLTLLGQQLLFPHATPVWIASM
FALGIGYLLPKRVLAAAAKHRQQTIAKEVSTFIPLLRILFESGMAVEQSLRVLSNEAQRL
LPALTHELRLILARVDSGLELGEELGKTARLLEVDEFTDTCIILQQLIHQGGGAMKSLLA
LKQLLDDRRLTRLQEYISKMSAKMSVVMMVFLFPALLIVLGGPAFIGIARALSHF