Protein Info for GFF6021 in Variovorax sp. SCN45

Annotation: Valyl-tRNA synthetase (EC 6.1.1.9)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 959 transmembrane" amino acids 499 to 518 (20 residues), see Phobius details TIGR00422: valine--tRNA ligase" amino acids 17 to 940 (924 residues), 1095.5 bits, see alignment E=0 PF00133: tRNA-synt_1" amino acids 33 to 626 (594 residues), 464 bits, see alignment E=1e-142 PF09334: tRNA-synt_1g" amino acids 63 to 209 (147 residues), 29.6 bits, see alignment E=7.7e-11 amino acids 355 to 567 (213 residues), 25.9 bits, see alignment E=1e-09 PF13603: tRNA-synt_1_2" amino acids 261 to 318 (58 residues), 27.5 bits, see alignment 5e-10 PF08264: Anticodon_1" amino acids 682 to 830 (149 residues), 137.5 bits, see alignment E=8.3e-44 PF10458: Val_tRNA-synt_C" amino acids 896 to 959 (64 residues), 68.2 bits, see alignment 1.5e-22

Best Hits

Swiss-Prot: 70% identical to SYV_CUPNJ: Valine--tRNA ligase (valS) from Cupriavidus necator (strain JMP 134 / LMG 1197)

KEGG orthology group: K01873, valyl-tRNA synthetase [EC: 6.1.1.9] (inferred from 79% identity to aav:Aave_1992)

Predicted SEED Role

"Valyl-tRNA synthetase (EC 6.1.1.9)" (EC 6.1.1.9)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.1.1.9

Use Curated BLAST to search for 6.1.1.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (959 amino acids)

>GFF6021 Valyl-tRNA synthetase (EC 6.1.1.9) (Variovorax sp. SCN45)
MSEPTTSPAQPGLDSLSKSFEPAAIEAHWGPEWEKRGYAKAGFRGTQQPKEGAESFAIQL
PPPNVTGTLHMGHAFNQTIMDSLTRYHRMKGANTLWVPGTDHAGIATQIVVERQLQEQKV
SRHELGRKNFVARVWEWKEKSGNTITQQMRRMGDTVDWSREYFTMDDDLSKVVMQTFVKL
YEEGLIYRGKRLGNWDPVLKTSVSDLEVESEEEDGFLWHLSYPLENGSGALTVATTRPET
MLGDTAVMVHPEDERYKHLIGQRVKLPLVGRLIPIIADDYVDKEFGTGVVKVTPAHDYND
YAVGQRHKLEMIGVLTLDATINDNAPEKYRGMDRFVARKAVVADLDALGLLVEVKKHKLM
VPRCARSGAIVEPMLTDQWYVAMTRPGADGQSIAQKAIDVVKSGEVRFVPENWVNTYNHW
MENIQDWTISRQLWWGHQIPAWYDEEGNVYVAQDEAAAQAKAPGKTLTRDEDVLDTWYSS
ALVPFSSLGWPEKTKDLELYLPSTVLVTGYDIIFFWVARMIMMTKHFTGKVPFRDVYIHG
LVRDAQGKKMSKSEGNVLDPVDLIDGIALPELLDKRSQGLRKPETAPTVRKNTQKEFPEG
IPAFGADALRFTFASLASLGRSINFDSKRCEGYRNFCNKLWNATRFVLMNCEGQDCGLLE
HTKEDCKVGGPAHGYLKFSRADYWIVSQLQRVEAEVTKGFDEYRLDNVANTIYQFAWDEF
CDWYLEIAKVQIQTGDDAQKRATRRTLIRVLETLLRLAHPVIPFITEELWQKVSKVAGRE
GESIMVAAYPQSQPEKIDEAAEAYVARLKALVDACRTLRGEMNVSPAMRLPLYAVADDAE
GAAFLREAAPVLQALAKLKEVKVFDDEASWQAAAEAAPVAVVGAARLCLHMEIDKAAEKL
RIGKEIARIEGEIAKVHGKLGNEAFVAKAPPAVIEQERKRLADFTATLERLRDQLVRLG