Protein Info for GFF60 in Sphingobium sp. HT1-2

Annotation: Pyruvate,phosphate dikinase (EC 2.7.9.1)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 899 TIGR01828: pyruvate, phosphate dikinase" amino acids 15 to 896 (882 residues), 1379.5 bits, see alignment E=0 PF01326: PPDK_N" amino acids 33 to 72 (40 residues), 33.9 bits, see alignment (E = 3.3e-12) amino acids 76 to 383 (308 residues), 153.7 bits, see alignment E=1.2e-48 PF00391: PEP-utilizers" amino acids 448 to 528 (81 residues), 86.5 bits, see alignment E=1.1e-28 PF02896: PEP-utilizers_C" amino acids 544 to 894 (351 residues), 264.5 bits, see alignment E=1.7e-82

Best Hits

Swiss-Prot: 64% identical to PPDK_RHIME: Pyruvate, phosphate dikinase (ppdK) from Rhizobium meliloti (strain 1021)

KEGG orthology group: K01006, pyruvate,orthophosphate dikinase [EC: 2.7.9.1] (inferred from 70% identity to azc:AZC_0410)

Predicted SEED Role

"Pyruvate,phosphate dikinase (EC 2.7.9.1)" in subsystem Glycolysis and Gluconeogenesis or Glycolysis and Gluconeogenesis, including Archaeal enzymes or Pyruvate metabolism I: anaplerotic reactions, PEP (EC 2.7.9.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.9.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (899 amino acids)

>GFF60 Pyruvate,phosphate dikinase (EC 2.7.9.1) (Sphingobium sp. HT1-2)
MVMTEEAQMSTTSTRYVYRFGGGVNDGGKGDKNLLGGKGANLDGMAAIGLPVPPGFTITT
EMCTRYYTDGGVYPESLKAEVANGIAHIEAVTGKKFGDAADPLLVSVRSGARISMPGMMD
TVLNLGLNDETVLGLAAASGDERFAWDSYRRFIQMYSDVVLELDHGAFEEALEIAKEDQG
YTLDTEMTADDWKALVSEYKALVAKLWNKPFPQDVADQLWGAISAVFGSWQADRAKVYRR
LNSIPGDWGTAVNVQAMVFGNMGDTSATGVAFTRDPATGENAYYGEYLINAQGEDVVAGI
RTPQYLTKQARERAGAKPLSMEEAMPETYAELARVFDILETHYRDMQDIEFTVQQGKLWM
LQTRSGKRTAKAALKIAVEMASEGLISEEEAVARVDPAALDQLLHPTLDPKAPRDVLTKG
LPASPGAASGAIVFDADTAERRNELGDAVILVRVETSPEDIHGMHAAKGILTARGGMTSH
AAVVARGMGRPCVSGAGSLSIDNANKILRIGSRELKEGDILTIDGATGEVMAGEVPTVQP
ELAGDFGILMAWADKVRRLKVRANAETPLDCQTARDFGAEGVGLCRTEHMFFDAARITAV
REMILADSEKGRRVALDKLLPEQRDDFAQIFMVMAGLPVTIRLLDPPLHEFLPHGEAEFE
EVAKAAGVGVEALKRRAAELHEFNPMLGHRGCRLGVTYPEIYEMQARAIFEAALIVKERS
GEAPIPEVMIPLVATKKELELMKAIVDQVAKDVFAEQGASVDYLVGTMIELPRAALKAGE
IAEVGEFFSFGTNDLTQTTIGISRDDAGRFLTQYVDKGIFARDPFVSIDVEGVGQLIELA
AERGRATRPGIKLGICGEHGGDPASIAFCEATGLDYVSASPYRVPIARLAAAQAALAKK