Protein Info for GFF5985 in Variovorax sp. SCN45

Annotation: dicarboxylic acid transporter PcaT

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 411 transmembrane" amino acids 12 to 29 (18 residues), see Phobius details amino acids 41 to 60 (20 residues), see Phobius details amino acids 72 to 91 (20 residues), see Phobius details amino acids 97 to 118 (22 residues), see Phobius details amino acids 139 to 159 (21 residues), see Phobius details amino acids 171 to 190 (20 residues), see Phobius details amino acids 221 to 239 (19 residues), see Phobius details amino acids 259 to 278 (20 residues), see Phobius details amino acids 289 to 308 (20 residues), see Phobius details amino acids 314 to 337 (24 residues), see Phobius details amino acids 348 to 370 (23 residues), see Phobius details amino acids 382 to 400 (19 residues), see Phobius details PF00083: Sugar_tr" amino acids 3 to 197 (195 residues), 88.1 bits, see alignment E=6.1e-29 amino acids 199 to 399 (201 residues), 42.3 bits, see alignment E=4.9e-15 TIGR00883: MFS transporter, metabolite:H+ symporter (MHS) family protein" amino acids 6 to 393 (388 residues), 466 bits, see alignment E=5.8e-144 PF07690: MFS_1" amino acids 32 to 264 (233 residues), 58.9 bits, see alignment E=4.2e-20 amino acids 234 to 401 (168 residues), 48.6 bits, see alignment E=5.9e-17

Best Hits

Swiss-Prot: 70% identical to KGTP_ECOLI: Alpha-ketoglutarate permease (kgtP) from Escherichia coli (strain K12)

KEGG orthology group: K03761, MFS transporter, MHS family, alpha-ketoglutarate permease (inferred from 97% identity to vpe:Varpa_1441)

MetaCyc: 70% identical to alpha-ketoglutarate:H+ symporter (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-23

Predicted SEED Role

"dicarboxylic acid transporter PcaT" in subsystem Protocatechuate branch of beta-ketoadipate pathway

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (411 amino acids)

>GFF5985 dicarboxylic acid transporter PcaT (Variovorax sp. SCN45)
MAASSGNLVEWFDFYVYAFSALYFAPAFFPKSDPTAQLLNTAGVFAAGFLMRPIGGWLFG
RVADRLGRKTSMLISVTMMCGGSLVIAALPTYAQIGAWAPFLLLVCRLFQGLSVGGEYGT
TATYMSEVALRGQRGFFSSFQYVTLIGGQLLAVLVIVALEQLLTEAELKAWGWRIPFVIG
AIAAVVALLLRRTLHETQSAEARENKEAGSIAGLFKHHTPAFLTVLGYTAGGSLIFYTFT
TYMQKYLVNTVHLPIKTASYVMTGALFVYMCMQPVFGALSDRIGRRNNMLLFGALGALTT
VPILTALQHTTSPVLAFVLIIVALAIVSFYTSISGIVKAEMFPPEVRALGVGLAYAVANA
IFGGSAEYVALGLKSLGHESAFFWYVSAMMVLAFVVSLRLPRQASYLHHDH