Protein Info for GFF5984 in Variovorax sp. SCN45

Annotation: L-lactate dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01070: FMN_dh
  • amino acids 17 to 380 (364 residues), 450.9 bits, E=4.5e-139
• PF03060: NMO
  • amino acids 252 to 363 (112 residues), 28.3 bits, E=1.8e-10

Best Hits

Swiss-Prot: 47% identical to LLDD_PSEU2: L-lactate dehydrogenase (lldD) from Pseudomonas syringae pv. syringae (strain B728a)

KEGG orthology group: K00101, L-lactate dehydrogenase (cytochrome) [EC: 1.1.2.3] (inferred from 94% identity to vpe:Varpa_1442)

MetaCyc: 44% identical to L-lactate dehydrogenase (Escherichia coli K-12 substr. MG1655)
1.1.5.M6 [EC: 1.1.5.M6]; RXN0-7227 [EC: 1.1.5.M6]

Predicted SEED Role

"L-lactate dehydrogenase (EC 1.1.2.3)" in subsystem L-rhamnose utilization or Lactate utilization or Respiratory dehydrogenases 1 (EC 1.1.2.3)

MetaCyc Pathways

• methylglyoxal degradation IV (3/3 steps found)
• methylglyoxal degradation V (3/3 steps found)
• L-lactaldehyde degradation (aerobic) (2/2 steps found)
• superpathway of methylglyoxal degradation (6/8 steps found)
• lactate fermentation to acetate, CO₂ and hydrogen (Desulfovibrionales) (3/8 steps found)
• superpathway of fucose and rhamnose degradation (4/12 steps found)

KEGG Metabolic Maps

• Pyruvate metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.1.2.3

Use Curated BLAST to search for 1.1.2.3 or 1.1.5.M6

Protein Sequence (385 amino acids)

>GFF5984 L-lactate dehydrogenase (Variovorax sp. SCN45)
VPDLSKITSIEDLRVIAKRRVPKMFYDYADSGAWTEGTYRANESDFQKIKLRQRVAVNME
GRSLRTTMVGQETAMPVAIAPTGLTGMQHADGEILGARAAKAFGIPFTLSTMSICSLEDI
AEHTGRHPFWFQLYVMKDRDFIERLIERAKAANVTALQLTLDLQILGQRHKDIKNGLTAP
PKPTIKNLINLATKPGWCMGMLGTKRRTFGNIAGHAKGVKDLSSLSSWTAEQFDPALSWA
DVEWIKKLWGGKLILKGIMDVEDARLAANSGADALIVSNHGGRQLDGAPSSVAALPAIAE
AVGSEIEVWMDGGIRSGQDVLKARALGARGTLIGRSFLYGLGAYGQAGVTRALQIIQKEM
DITMAFCGRTNIDDVDSSILLPGTF