Protein Info for GFF596 in Sphingobium sp. HT1-2

Annotation: Ribonuclease E (EC 3.1.26.12)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 908 TIGR00757: ribonuclease, Rne/Rng family" amino acids 14 to 505 (492 residues), 526.1 bits, see alignment E=3.2e-162 PF10150: RNase_E_G" amino acids 208 to 478 (271 residues), 332.5 bits, see alignment E=3.1e-103 PF20833: RNase_E_G_Thio" amino acids 490 to 573 (84 residues), 87.8 bits, see alignment E=6.4e-29

Best Hits

KEGG orthology group: K08300, ribonuclease E [EC: 3.1.26.12] (inferred from 81% identity to sjp:SJA_C1-07660)

Predicted SEED Role

"Cytoplasmic axial filament protein CafA and Ribonuclease G (EC 3.1.4.-)" in subsystem Bacterial Cell Division or RNA processing and degradation, bacterial (EC 3.1.4.-)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.1.26.12

Use Curated BLAST to search for 3.1.26.12 or 3.1.4.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (908 amino acids)

>GFF596 Ribonuclease E (EC 3.1.26.12) (Sphingobium sp. HT1-2)
MTMRMLIDARHREETRVAVVKGNRIEEFDFESAEHKQLKGNIYLAKVTRVEPSLQAAFVD
YGGNRHGFLAFSEIHPDYYQIPREDREALLREERAHAEEEAALRADYDEDEDAAGDDGVE
VVEATHHDDEEGAHEAEASEGESNEGGRPNRRKREKGDDAADELRRKRMALRRRYKIQDV
IKRRQVLLVQVVKEERGNKGAALTTYLSLAGRYCVLMPNTSHGGGISRKISNAADRKRLK
SIIAEMALPSSMGCIVRTAGLQRTKPEIKRDFDYLARLWDEIREKTLRAAAPELIHNDSD
LIKRAIRDIYNKDIEEVIVEGEHGYKAAKDFMKLLMPSHARRVKQYADAVSLFQRASVED
QLAAMYNPVVQLKSGGYLVINPTEALVSIDINSGRSTREHGIEQTAVATNLEAAREIARQ
LRLRDMAGLVVIDFIDMEMNSNIRKVEKAMKEALKDDRARIQVGRISGFGLMEMSRQRLR
TGVLEASTRQCPHCEGTGLVRTASSAGLGALRMLEEEAARGRGSVITLRASQEAAFYVLN
NKRRELDEIEQRYGVRIVVLPDGEIEGARMSVEPSGPRPERVVTYAPLAEEEDDLDVIED
EEEEEEIEEEAAPERQERGERGDRGEEREGGRRRRRRRRRRGGQRDDAPGETGEDNGAED
GEEGEESAEIETDAEAVAAEPAAEGEDEGGSRRRGRRGRRGGRRRREGGEAGAEAGETAG
DDSAETVIEAEAAAPVEEAPVAEAAPEEAPAEEAPKTRRRPRARKAKDAAAEPAAVEVTE
ALAEVVAEAPAAEPVVEEAAEKPKPKRTRKKKADVEAEAAAAAEAPAVEVVAEEAAEKPK
PKRTRKKKVEPVAEEVAVEAAPAVTEVAAAPAEAAPASEAVTADPAAEVESDENGEPRRG
WWQRTFGQ